PXD057532

PXD057532 is an original dataset announced via ProteomeXchange.

Title	Comparative analysis of metabolic and functional cardiac alterations in diet- and genetically induced mouse models of heart failure
Description	This study aimed to elucidate the metabolic and functional alterations in heart failure in two mouse models with different heart failure phenotypes. C57BL/6J mice fed a high-fat diet (Black6 HF) and New Zealand Obese mice fed a standard diet (NZO SD) were analyzed compared to Black6 SD controls. Functional parameters of the heart were assessed through echocardiography. Additionally, plasma metabolites and tissue fibrosis were analyzed. Proteomics data from heart tissue were incorporated into a detailed kinetic model of central cardiac metabolism (CARDIOKIN1) to evaluate metabolic capacities. We demonstrate distinct structural and functional cardiac impairments, nutritional imbalances, and metabolic adaptations. Black6 HF mice exhibited cardiac dysfunction with normal ejection fraction, and decreased cardiac mass and inflammation, but without fibrosis while maintaining stable plasma nutrient composition. In contrast, NZO SD mice displayed heart failure with reduced ejection fraction, increased heart mass, significant derangement of circulating plasma nutrients, and mild fibrosis, but no inflammation. Proteomic analysis and kinetic modeling revealed that mitochondrial dysfunction and reduced ATP production capacity are key drivers of heart failure in Black6 HF mice, while NZO SD mice maintained ATP production capacity but showed significant alterations in substrate utilization capacities under variable energy demands. Our findings highlight the critical role of metabolic adaptation in heart failure emphasizing the importance of metabolic flexibility and efficiency. This study underscores the complex interplay between metabolic, structural, and functional changes in heart failure, providing valuable insights into and underpinning the different basis for treatment strategies for different forms of this syndrome.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_21:25:59.581.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christoph Gille
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-05 05:34:32	ID requested
⏵ 1	2026-04-26 21:26:00	announced

10.1111/febs.70362;

Ott C, Baumgarten P, Henning T, Weber D, Wallach I, Grune J, Kintscher U, Holzh, ü, tter HG, Grune T, Berndt N, Comparative analysis of metabolic and functional cardiac alterations in diet- and genetically induced mouse models of cardiac dysfunction. FEBS J, 293(8):2454-2477(2026) [pubmed]

submitter keyword: High-fat diet, cardiac metabolism, genetically induced obesity, heart failure, metabolic adaptation, cardiac function

Nicolaus Berndt
contact affiliation	Deutsches Herzzentrum der Charité (DHZC), Institute of Computer-assisted Cardiovascular Medicine
contact email	nikolaus.berndt@dhzc-charite.de
lab head
Christoph Gille
contact affiliation	Core Facility -High-Throughput Mass Spectrometry, Charité
contact email	christoph.gille@charite.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD057532

PRIDE project URI

• PRIDE
  1. PXD057532
     1. Label: PRIDE project
     2. Name: Comparative analysis of metabolic and functional cardiac alterations in diet- and genetically induced mouse models of heart failure