PXD057503

PXD057503 is an original dataset announced via ProteomeXchange.

Title	COG complex-dependent Golgi-derived vesicles from hTERT-RPE1 cells
Description	Intracellular trafficking relies on small vesicular intermediates, though their specific role in Golgi function is still debated. To clarify this, we induced acute dysfunction of the Conserved Oligomeric Golgi (COG) complex and analyzed vesicles from cis, medial, and trans-Golgi compartments. Proteomic analysis of Golgi-derived vesicles from wild-type cells revealed distinct molecular profiles, indicating a robust recycling system for Golgi proteins. Notably, these vesicles retained various vesicular coats, while COG depletion accelerated uncoating. The increased overlap in molecular profiles with COG depletion suggests that persistent defects in vesicle tethering disrupt intra-Golgi sorting. Our findings reveal that the entire Golgi glycosylation machinery recycles within vesicles in a COG-dependent manner, whereas secretory and ER-Golgi trafficking proteins were not enriched. These results support a model in which the COG complex orchestrates multi-step recycling of glycosylation machinery, coordinated by specific Golgi coats, tethers, Rabs, and SNAREs.
HostingRepository	PRIDE
AnnounceDate	2026-08-31
AnnouncementXML	Submission_2026-08-30_16:35:03.816.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vladimir Lupashin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-04 17:04:40	ID requested
⏵ 1	2026-08-30 16:35:04	announced

10.1091/mbc.e24-12-0556;

Sumya FT, Aragon-Ramirez WS, Lupashin VV, Deep proteomic profiling of the intra-Golgi trafficking intermediates. Mol Biol Cell, 36(7):ar87(2025) [pubmed]

submitter keyword: Golgi, BET1L, vesicle, COG, immunoprecipitation, GOLGA5,human, GOLGB1

Vladimir Lupashin
contact affiliation	Department of Physiology and Cell Biology, University of Arkansas for Medocal Sciences, AR, USA
contact email	vvlupashin@uams.edu
lab head
Vladimir Lupashin
contact affiliation	University of Arkansas for Medical Scinces
contact email	vvlupashin@uams.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD057503

PRIDE project URI

• PRIDE
  1. PXD057503
     1. Label: PRIDE project
     2. Name: COG complex-dependent Golgi-derived vesicles from hTERT-RPE1 cells