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PXD057479
PXD057479 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | O-GlcNAcylation of BRD4 attenuates phase separation to regulate transcription |
| Description | BRD4 undergoes liquid-liquid phase separation (LLPS) at super-enhancers (SEs) and confers prominent gene control. However, the regulatory mechanism and transcriptional consequences of BRD4 condensation remain elusive. Here we identified that the C-terminal intrinsic disordered region of BRD4 serves as the primary interacting region with O-GlcNAc transferase (OGT) for O-GlcNAc modifications at 12 possible sites. O-GlcNAcylation attenuates the LLPS propensity of BRD4 with reduced puncta size and increased fluidity. These in turn result in significantly declined occupancy and transcription at SEs. We further demonstrated that loss of O-GlcNAcylation on BRD4 led to elevated gene expression relating to cell cycle and DNA damage, allowing cells less sensitive to DNA damage and grow faster. Therefore, our results demonstrate an O-GlcNAc–mediated regulatory mechanism of BRD4 LLPS for gene control at SEs, showcasing an example of how post-translational modifications regulate transcriptional activities at SEs through a phase separation model. |
| HostingRepository | iProX |
| AnnounceDate | 2025-06-11 |
| AnnouncementXML | Submission_2026-07-24_02:23:18.133.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yun Ge |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos; Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-11-03 23:54:43 | ID requested | |
| ⏵ 1 | 2026-07-24 02:23:18 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: O-GlcNAc, BRD4 |
Contact List
| Yun Ge | |
|---|---|
| contact affiliation | Shenzhen Bay Laboratory |
| contact email | geyun@szbl.ac.cn |
| lab head | |
| Yun Ge | |
| contact affiliation | Shenzhen Bay Laboratory |
| contact email | geyun@szbl.ac.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
