PXD057462

PXD057462 is an original dataset announced via ProteomeXchange.

Title	Macrophage migration inhibitory factor in inflammasome formation and macrophage recruitment in cervical cell carcinoma
Description	Despite the demonstrated efficacy of immune checkpoint blockade therapies in various tumors, their efficacy in cervical cancer is limited by factors such as the need for positive PD-L1 expression and the development of drug resistance. A crucial pro-inflammatory cytokine, macrophage migration inhibitory factor (MIF), is highly expressed in various cancers and contributes to tumor progression by regulating inflammatory responses and the tumor microenvironment. The present study aimed to explore the role of macrophage migration inhibitory factors in cervical squamous cell carcinoma (CSCC) recurrence and metastasis. Western blotting, reverse transcription quantitative PCR, cell-counting kit 8 assay, flow cytometry, and ELISA were used to investigate the effects of MIF on CSCC progression and formation of inflammasomes. Transcriptome and proteome sequencing were combined to screen for key effector proteins of MIF. Moreover, in vitro co-culture experiments, Transwell assays, and flow cytometry were used to evaluate the roles of MIF and TSC22 domain family protein 3 (TSC22D3) as inflammatory tumor-promoting factors in macrophage recruitment and polarization induction. The results indicated that MIF was highly expressed in CSCC with lymph node metastasis, positively correlated with cervical cancer stage, and associated with poor prognosis. MIF was found to promote CSCC progression and linked to inflammasome activation. Multi-omics screening results indicated that TSC22D3 may be a crucial interacting gene of MIC. Moreover, MIF and TSC22D3 could facilitate inflammasome activation, macrophage recruitment, and M2 polarization. Therefore, MIF and TSC22D3 can induce macrophage infiltration in cervical cancer lesions and affect the tumor microenvironment by polarizing macrophages toward the M2 phenotype, promoting CSCC progression. This study highlights the potential of targeting the MIF-TSC22D3 axis as a novel therapeutic strategy and offering a promising avenue for improving the efficacy of immunotherapy in treatin
HostingRepository	PRIDE
AnnounceDate	2025-01-26
AnnouncementXML	Submission_2025-01-26_05:25:17.371.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	qianqian zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-03 04:04:07	ID requested
⏵ 1	2025-01-26 05:25:17	announced

10.3892/OL_XXXXXXXX;

submitter keyword: CSCC, TMT, PD-L1,human

qianqian zhang
contact affiliation	Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital
contact email	zhangqianqian2@sxmu.edu.cn
lab head
qianqian zhang
contact affiliation	Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital
contact email	zhangqianqian2@sxmu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057462
     1. Label: PRIDE project
     2. Name: Macrophage migration inhibitory factor in inflammasome formation and macrophage recruitment in cervical cell carcinoma