PXD057461

PXD057461 is an original dataset announced via ProteomeXchange.

Title	The protein interactome of the human C3orf33
Description	The protein interactome of the human C3orf33 protein was explored in U2OS cells through affinity purification-mass spectrometry (AP-MS). Specifically, U2OS cells stably expressing C3orf33 with either an N-terminus or a C-terminus 3XFlag tag were initially lysed using RIPA buffer. Subsequently, the proteins associated with C3orf33 were purified with anti-flag magnetic beads. Proteins were then eluted from the beads using competition with 3×Flag peptides and subsequently analyzed with mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2025-08-23
AnnouncementXML	Submission_2025-08-22_17:52:46.131.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yue Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-03 02:00:39	ID requested
⏵ 1	2025-08-22 17:52:46	announced

10.1101/2025.01.05.631328;

submitter keyword: LC-MSMS,Human，C3orf33

Li He
contact affiliation	Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230000, China
contact email	lihe19@ustc.edu.cn
lab head
Yue Zhang
contact affiliation	University of Science and Technology of China
contact email	zylcd@ustc.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD057461

PRIDE project URI

• PRIDE
  1. PXD057461
     1. Label: PRIDE project
     2. Name: The protein interactome of the human C3orf33