PXD057448

PXD057448 is an original dataset announced via ProteomeXchange.

Title	Activity-dependent COX-2 proteolysis
Description	Cyclooxygenase-2 (COX-2) catalyzes the oxidation of arachidonic acid (AA) into a single product that is the source of all prostaglandins (PGs), ligands of multiple pro-inflammatory pathways. AA catalysis results in suicide inactivation, rendering the enzyme catalytically inactive. We report that catalytic activity also leads to controlled cleavage of COX-2, an event that is differentially regulated by fatty acids, and blocked by COX inhibitors. Using mass spectrometry, we identified two adjacent cleavage points within the catalytic domain, which give rise to COX-2 fragments that are catalytically inactive and localize to different cellular compartments. These fragments were also detected in human colon tumors. Expression of one of these fragments in cells significantly reduced mitochondrial function, increased lactate production, and enhanced proliferation.
HostingRepository	PRIDE
AnnounceDate	2024-11-25
AnnouncementXML	Submission_2024-11-25_04:17:06.524.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oded Kleifeld
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-02 07:10:21	ID requested
⏵ 1	2024-11-25 04:17:06	announced

10.1016/J.ISCI.2024.111403;

submitter keyword: proteolysis,Cyclooxygenase-2, arachidonic acid

Liza Barki-Harrington
contact affiliation	Department of Human Biology, Faculty of Natural Sciences, University of Haifa, Haifa, 3103301 Israel
contact email	Lbarki@psy.haifa.ac.il
lab head
Oded Kleifeld
contact affiliation	Technion
contact email	okleifeld@technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD057448

PRIDE project URI

• PRIDE
  1. PXD057448
     1. Label: PRIDE project
     2. Name: Activity-dependent COX-2 proteolysis