PXD057444

PXD057444 is an original dataset announced via ProteomeXchange.

Title	Ionizable polymeric micelles (IPMs) for efficient siRNA delivery
Description	Lipid nanoparticles (LNPs) are widely used for nucleic acid delivery but face challenges like limited targeting and accelerated blood clearance. We design three ionizable oligo-mers (IOs) that, with PLA-PEG, form Ionizable Polymeric Micelles (IPMs), a potential siRNA delivery system, which can achieve lysosomal escape. FAPi-modified IPMs (FAPi-IPMs) show higher targeting of activated hepatic stellate cells (HSCs) compared to hepatocytes, silencing both HSP47 and HMGB1, reducing collagen secretion and liv-er inflammation, effectively treating fibrosis. Moreover, IPMs and FAPi-IPMs mitigate accelerated blood clearance and produce fewer PEG antibodies than LNPs. Unlike LNPs, which adsorb apolipoprotein E (ApoE), IPMs and FAPi-IPMs show minimal apolipoprotein adsorption, differentiating their targeting effects from LNPs. In conclu-sion, IPMs represent an alternative nucleic acid delivery system with superior targeting and reduced immune clearance.
HostingRepository	PRIDE
AnnounceDate	2024-11-21
AnnouncementXML	Submission_2024-11-21_00:45:33.403.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ziyu Zhou
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-01 21:04:37	ID requested
⏵ 1	2024-11-21 00:45:33	announced

Dataset with its publication pending

submitter keyword: HSP47, protein corona, HMGB1, hepatic fibrosis, fibroblast activation protein inhibitor (FAPi), nucleic acid delivery,Ionizable polymeric micelles (IPMs), hepatic stellate cells

Zhiqiang Yan
contact affiliation	Institute of Biomedical Engineering and Technology, Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200062, P.R. China
contact email	zqyan@sat.ecnu.edu.cn
lab head
Ziyu Zhou
contact affiliation	East China Normal University
contact email	zyzhou99@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD057444

PRIDE project URI

• PRIDE
  1. PXD057444
     1. Label: PRIDE project
     2. Name: Ionizable polymeric micelles (IPMs) for efficient siRNA delivery