PXD057441

PXD057441 is an original dataset announced via ProteomeXchange.

Title	Computationally-aided and polyketide synthase-based controlled synthesis of polycyclopropanated fuel molecules
Description	Reducing carbon emissions from aviation and other long-distance transportation sectors requires the development of sustainable biofuels with suitable energy density, freezing point, and other physical properties. We previously demonstrated heterologous production of high energy polycyclopropanated fatty acids (POP-FAs, class I) using an iterative polyketide synthase (iPKS) pathway in a Streptomyces host. This pathway naturally incorporates multiple cyclopropane rings in a manner that is challenging to replicate with organic synthesis. However, to engineer biosynthesis towards specific desired POP-FA molecules, the product programming mechanisms for this iterative pathway must be understood. Here we present a strategy to modify the chain length and cyclopropane ratio of POP-FAs by in vivo gene exchange. Guided by phylogenetic and structural analysis, we identify the POP cyclopropanase (CP) gene to be a key target for POP diversification and engineering. Then, leveraging both natural and engineered pathway product diversity, we demonstrate targeted production of new classes of POP-FAs, namely medium-chain POP-FAs (class II) with distinct cyclopropane positions, as well as fully cyclopropane-saturated POP-FAs (class III) by chimeric POP iPKS pathways in Streptomyces. Compared to class I POP-FAs, class II POP-FAs have superior freezing point properties for aviation, while class III POP-FAs present an energy-density improvement for rocketry. These precise and controllable modifications to POP-FA structure open the door for bioproduction of designer POP fuels.
HostingRepository	PRIDE
AnnounceDate	2026-03-05
AnnouncementXML	Submission_2026-03-05_11:01:35.388.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Petzold
SpeciesList	scientific name: Streptomyces albus group; NCBI TaxID: NEWT:1852274; scientific name: Streptomyces coelicolor; NCBI TaxID: NEWT:1902;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-01 16:28:32	ID requested
⏵ 1	2026-03-05 11:01:35	announced

Dataset with its publication pending

submitter keyword: polyketide synthase,polycyclopropanated fatty acids,biosynthetic engineering

Christopher J. Petzold
contact affiliation	Staff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720
contact email	cjpetzold@lbl.gov
lab head
Christopher Petzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	cjpetzold@lbl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057441
     1. Label: PRIDE project
     2. Name: Computationally-aided and polyketide synthase-based controlled synthesis of polycyclopropanated fuel molecules