PXD057436

PXD057436 is an original dataset announced via ProteomeXchange.

Title	Proteomics on visceral adipose tissue derived extracellular vesicles from Tsg101 knockout mice
Description	The protein Tsg101 plays an integral role extracellular vesicle formation and secretion from cells. We developed a mouse model with Tsg101 knockdown (Tsg101dAd). Visceral adipose tissue (VAT) was harvested from Tsg101dAD and Tsg101flfl mice fed high-fat diet to induce obesity or matched diet as non-obese controls. VAT was ex vivo cultured for 48 hrs and its conditioned media was collected. Extracellular vesicles were isolated from conditioned media by ultracentrifugation. The aim of the project was to characterize extracellular vesicle protein cargo from obese and non-obese VAT.
HostingRepository	PRIDE
AnnounceDate	2025-04-22
AnnouncementXML	Submission_2025-04-22_09:21:58.031.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Parsa Haque
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-01 10:44:35	ID requested
⏵ 1	2025-04-22 09:21:58	announced

Haque PS, Goodman D, Kuusivuori-Robinson T, Coughlan C, Delgado-Deida Y, Onyiah JC, Zempleni J, Theiss AL, -oxidation to Drive Colonic Stemness. Cell Mol Gastroenterol Hepatol, 19(7):101504(2025) [pubmed]

10.1016/j.jcmgh.2025.101504;

submitter keyword: Adipose tissue, extracellular vesicles, Tsg101

Arianne Theiss
contact affiliation	Division of Gastroenterology and Hepatology, School of Medicine, University of Colorado Anschutz Medical Campus,Colorado, USA
contact email	arianne.theiss@cuanschutz.edu
lab head
Parsa Haque
contact affiliation	University of Colorado Anschutz Medical Campus
contact email	parsa.haque@cuanschutz.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD057436

PRIDE project URI

• PRIDE
  1. PXD057436
     1. Label: PRIDE project
     2. Name: Proteomics on visceral adipose tissue derived extracellular vesicles from Tsg101 knockout mice