PXD057430

PXD057430 is an original dataset announced via ProteomeXchange.

Title	A0913 interacts with CP47 of PSII and ApcF of PBS
Description	Co-immunoprecipitation (CoIP) was performed to identify the proteins that A0913 interacts with. The thylakoid membranes were solubilized with the detergent n-Dodecyl-β-D-maltoside (DDM) before CoIP was performed using the monoclonal antibodies against the Flag tag to precipitate the target proteins. Four proteins were obtained by the CoIP as revealed by SDS-PAGE analysis . Immunoblotting with antibodies against CP47 and the Flag tag revealed that the top band in the gel was CP47 while the third band from the top down was A0913CF . Mass spectrometry revealed that these bands were CP47 of PSII。
HostingRepository	PRIDE
AnnounceDate	2024-11-06
AnnouncementXML	Submission_2024-11-06_05:58:00.750.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhenggao Zheng
SpeciesList	scientific name: Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum); NCBI TaxID: 32049;
ModificationList	O-phosphopantetheine-L-serine; acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-01 08:02:11	ID requested
⏵ 1	2024-11-06 05:58:01	announced

Dataset with its publication pending

submitter keyword: CO-IP,0913-CF,WT,LC-MSMS,CP47

Jindong Zhao
contact affiliation	State Key Laboratory of Protein and Plant Genetic Engineering, School of Life Science, Peking University, Beijing, 100871, P. R. China.
contact email	jzhao@pku.edu.cn
lab head
Zhenggao Zheng
contact affiliation	Peking University
contact email	zhengzhenggao@pku.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD057430

PRIDE project URI

• PRIDE
  1. PXD057430
     1. Label: PRIDE project
     2. Name: A0913 interacts with CP47 of PSII and ApcF of PBS