PXD057429

PXD057429 is an original dataset announced via ProteomeXchange.

Title	Heterologous production of phosphorylated casein
Description	The demand for milk proteins is on the rise, but sustainability concerns dictate a reduction of animal-based agriculture. Microbial cell factories can be a viable alternative, but their inability to phosphorylate recombinant caseins is a major bottleneck, since phosphorylation is needed for calcium binding and functionality. We propose a solution based on engineered bacterial protein kinases, which results in microbially produced caseins with the correct phosphorylation pattern and functional properties.
HostingRepository	PRIDE
AnnounceDate	2025-07-04
AnnouncementXML	Submission_2025-07-04_08:15:24.662.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carsten Jers
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-01 07:24:53	ID requested
⏵ 1	2025-07-04 08:15:25	announced

10.1016/J.TIBTECH.2025.05.015;

submitter keyword: E. coli,casein, phosphorylation

Ivan Mijakovic
contact affiliation	Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark and Systems and Synthetic Biology Division, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden
contact email	ivan.mijakovic@chalmers.se
lab head
Carsten Jers
contact affiliation	DTU Biosustain, Technical University of Denmark
contact email	cjer@dtu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD057429

PRIDE project URI

• PRIDE
  1. PXD057429
     1. Label: PRIDE project
     2. Name: Heterologous production of phosphorylated casein