PXD057367

PXD057367 is an original dataset announced via ProteomeXchange.

Title	The infThe inflammatory microenvironment changes the biological function of sEV by modifying the composition of their protein coronalammatory microenvironment changes the biological function of sEV by modifying the composition of their protein corona
Description	A relevant part of the physiological functions of extracellular vesicles is conditioned by the protein corona attached to the surface. The composition of this corona is initially defined during their intracellular synthesis, but it can be modified subsequently by the interactions with the microenvironment. Here we evaluated how the corona of small extracellular vesicles exposed to the inflammatory environment generated in acute pancreatitis is modified and which functional changes their undergo as a result of these modifications. Small extracellular vesicles obtained from a pancreatic cell line were incubated with the ascitic fluid generated in experimental acute pancreatitis in rats. By proteomic techniques, we have detected the appearance of new proteins and the redistribution of some others already present. These changes increase the uptake of extracellular vesicles by some cell types and the response induced in inflammatory cells. The inhibition of different Pattern recognition receptors reversed this activation, indicating that some of these effects could be due to binding of Damage-associated molecular patterns to the corona. All this indicates that in pathologies such as acute pancreatitis, with an inflammatory response and intense tissue damage, the microenvironment substantially modifies the corona of extracellular vesicles, thus altering their behavior and enhancing their inflammatory activity.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_04:27:53.508.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Montserrat Carrascal
SpeciesList	scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-30 13:19:54	ID requested
⏵ 1	2025-05-07 04:27:54	announced

Armengol-Badia O, Maggi J, Casal C, Cort, é, s R, Abi, á, n J, Carrascal M, Closa D, The Microenvironment in an Experimental Model of Acute Pancreatitis Can Modify the Formation of the Protein Corona of sEVs, with Implications on Their Biological Function. Int J Mol Sci, 25(23):(2024) [pubmed]

10.3390/ijms252312969;

submitter keyword: exosomes

corona

inflammation

pancreatitis

extracellular vesicles

Daniel Closa
contact affiliation	Department of Experimental Pathology, Institut d’Investigacions Biomèdiques de Barcelona, Consejo Superior de Investigaciones Científicas (IIBB-CSIC), Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
contact email	daniel.closa@iibb.csic.es
lab head
Montserrat Carrascal
contact affiliation	IIBB-CSIC
contact email	montserrat.carrascal.csic@uab.cat
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD057367

PRIDE project URI

• PRIDE
  1. PXD057367
     1. Label: PRIDE project
     2. Name: The infThe inflammatory microenvironment changes the biological function of sEV by modifying the composition of their protein coronalammatory microenvironment changes the biological function of sEV by modifying the composition of their protein corona