PXD057359

PXD057359 is an original dataset announced via ProteomeXchange.

Title	tRNA as an assembly chaperone for a macromolecular transcription-processing complex
Description	tRNAs are widely recognized for their role in translation. Here we describe a previously unidentified function of tRNA as an assembly chaperone. During poxviral infection tRNAGln/Arg lacking the anticodon mcm5s2U modification is specifically recruited from the cellular tRNA pool to a multi-subunit poxviral RNA polymerase complex (vRNAP), where it controls the transition to the pre-initiation complex upon infection. Cryo-EM analysis of assembly intermediates illustrates how tRNAGln/Arg orchestrates the recruitment of transcription and mRNA processing factors to vRNAP. The atomic vRNAP structures reveal an induced-fit mechanism that internalizes anticodon base G36 into the anticodon stem creating a non-canonical tRNA structure and selecting a defined tRNA modification pattern. The role of tRNA as assembly chaperone extends to the pathogenic MPXV due to the high conservation of vRNAP.
HostingRepository	PRIDE
AnnounceDate	2025-11-24
AnnouncementXML	Submission_2025-11-23_16:19:31.133.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Bender
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Vaccinia virus GLV-1h68; NCBI TaxID: NEWT:502057;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-30 08:23:19	ID requested
⏵ 1	2025-11-23 16:19:32	announced

Bartuli J, Jungwirth S, Dixit M, Okuda T, Zimmermann JP, Erlacher M, Pan T, Volz A, H, ü, ttenhofer A, Warscheid B, H, ö, bartner C, Grimm C, Fischer U, tRNA as an assembly chaperone for a macromolecular transcription-processing complex. Nat Struct Mol Biol, 32(11):2349-2358(2025) [pubmed]

10.1038/s41594-025-01653-y;

submitter keyword: AP-MS,FLAG-IP, Vaccina virus

Bettina Warscheid
contact affiliation	Chair of Biochemistry II, Theodor Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany
contact email	bettina.warscheid@uni-wuerzburg.de
lab head
Julian Bender
contact affiliation	University of Wuerzburg Chair of Biochemistry II Am Hubland 97074 Würzburg
contact email	julian.bender@uni-wuerzburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD057359

PRIDE project URI

• PRIDE
  1. PXD057359
     1. Label: PRIDE project
     2. Name: tRNA as an assembly chaperone for a macromolecular transcription-processing complex