PXD057348

PXD057348 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of extracellular vesicles identifies CDCP1 as critical metastasis-related glycoprotein in lung cancer
Description	Lung cancer is the most prevalent malignancy worldwide, with metastasis causing over 90% of fatalities. Recent studies have demonstrated the pivotal role of extracellular vesicles (EVs) and glycoproteins in tumor progression. In this study, we compared the glycoproteome of EVs from 95C (low metastatic) and 95D (high metastatic) lung cancer cells to find key targets in metastasis. Through coupling lectin affinity chromatography with quantitative proteomics, 1562 glycoproteins were identified. Compared to 95C EVs, 23 glycoproteins exhibited a more than 20-fold increase in 95D EVs, including CDCP1, TNC, NCAM2, and ITGA4. CUB-domain containing protein 1 (CDCP1) was upregulated 143-fold in 95D EVs, which is significantly correlated with poor prognosis of lung cancer patients in the TCGA database. We then generated CDCP1 knockout (KO) 95D cell lines and revealed that the absence of CDCP1 reduced cell migration ability, which was also confirmed by EVs and cell co-culture experiments. We further performed Ti4+-IMAC based phosphoproteomic analysis to investigate the changes of signaling pathways in CDCP1 KO cell lines. 147 differentially expressed phosphoproteins were revealed, which involve in cell adhesion, cytoskeletal regulation, and ErbB signaling. Verification experiments confirmed that the levels of phosphorylated SRC and JUN proteins, markers of ErbB signaling pathway, were decreased 5.5-fold and 4.2-fold, respectively, indicating that CDCP1 can promote cell migration through the ErbB signaling pathway. Our data demonstrate that glycoprotein CDCP1 was selectively packed into EVs and potentially contributed to cancer metastasis, which is a critical target for anti-metastasis research and drug discovery.
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-03_16:24:31.683.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lu Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-30 03:40:45	ID requested
⏵ 1	2025-08-03 16:24:32	announced

10.1002/jev2.70128;

Zhang L, Wu Y, Li S, Guo M, Zhao J, Cao C, Zhang Y, Xiao H, Proteomic Analysis of Extracellular Vesicles Identifies CDCP1 as Critical Metastasis-Related Glycoprotein in Lung Cancer. J Extracell Vesicles, 14(7):e70128(2025) [pubmed]

submitter keyword: CDCP1, Extracellular Vesicles, Proteomics, Lung Cancer

Hua Xiao
contact affiliation	State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
contact email	huaxiao@sjtu.edu.cn
lab head
Lu Zhang
contact affiliation	State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
contact email	zhanglu-sjtu@sjtu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057348
     1. Label: PRIDE project
     2. Name: Proteomic analysis of extracellular vesicles identifies CDCP1 as critical metastasis-related glycoprotein in lung cancer