PXD057346

PXD057346 is an original dataset announced via ProteomeXchange.

Title	Sitagliptin attenuates L-dopa-induced dyskinesia by regulating mitochondrial function and neuronal activity in 6-OHDA-induced mouse model of Parkinson’s disease
Description	L-dopa-induced dyskinesia (LID) is an incapacitating complication of long-term administration of L-dopa therapy that commonly affects patients with Parkinson’s disease (PD) due to the widespread use of the causative drug. Herein, we investigated the therapeutic potential of sitagliptin, a drug used to treat type 2 diabetes mellitus, to treat LID. 6-hydroxydopamine (6-OHDA) was unilaterally injected into the left side of the substantia nigra pas compacta to induce a mouse model of PD. After four weeks of 6-OHDA induction, L-dopa was administered with or without sitagliptin for 11 consecutive days. LID was monitored using abnormal involuntary movement (AIM) scoring, conducted on days 5 and 10 of L-dopa treatment. Comparative proteomic analysis was performed on the 6-OHDA-lesioned striatum by comparing groups treated with vehicle + L-dopa and sitagliptin + L-dopa. Changes in the expression of Ndufb2 and Arc, which showed the greatest increase or decrease, were validated using western blotting and RT-PCR analysis. Sitagliptin combined with L-dopa significantly attenuated AIM scores in 6-OHDA-lesioned mice. The protein level of Ndufb2 was increased by the co-administration of sitagliptin and L-dopa in both unlesioned and 6-OHDA-lesioned striata compared with the administration of L-dopa alone. The expression levels of FosB and c-Fos, and Arc were suppressed by the co-administration of sitagliptin and L-dopa in the 6-OHDA-lesioned striatum. These findings suggest that sitagliptin may have therapeutic potential for incapacitating complications of long-term L-dopa use in patients with PD.
HostingRepository	PRIDE
AnnounceDate	2026-05-20
AnnouncementXML	Submission_2026-05-20_03:16:13.943.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ga Seul Lee
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-30 01:59:38	ID requested
⏵ 1	2026-05-20 03:16:14	announced

10.1007/S00702-025-02907-1;

submitter keyword: proteomics, striatum, abnormal involuntary movement, Arc, Ndufb2, FosB/ΔFosB

Kyoung-Shim Kim
contact affiliation	Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125, Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
contact email	kskim@kribb.re.kr
lab head
Ga Seul Lee
contact affiliation	Korea Research Institute of Bioscience & Biotechnology (KRIBB)
contact email	rktmf1013@kribb.re.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057346
     1. Label: PRIDE project
     2. Name: Sitagliptin attenuates L-dopa-induced dyskinesia by regulating mitochondrial function and neuronal activity in 6-OHDA-induced mouse model of Parkinson’s disease