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PXD057344

PXD057344 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThiol metabolic heterogeneity in immune cells causes distinct molecular mechanisms of toxicity upon chronic lead exposure
DescriptionLead pollution is global, billions of people are chronically exposed to lead. Biological thiols are nature sequesters of lead. Human immune system is highly vulnerable to lead, unfortunately the underlying mechanism remains unknown. Using mass cytometry and mass spectrometry, we systematically identified lead targets in immune cells from mice model of chronic lead exposure. We found CD4+ T and neutrophil are most vulnerable to lead, respectively due to low thiol and high glutathione metabolism. Specifically, in CD4+ T, lack of thiol renders severe damage of ribosome by lead, which we successfully rescued using thiol drugs. In neutrophil, robust glutathione metabolism accumulates lead, which can be passed on to proteins of higher binding affinity. Among these proteins, our evidences suggest lead suppresses immune activation through PKC-like, PE/DAG-binding domain on RAF1 and PKCs. Overall, it clarified the mechanisms of toxicity in immune cells upon chronic lead exposure, which provides valuable information for medical care and public health.
HostingRepositoryiProX
AnnounceDate2024-10-30
AnnouncementXMLSubmission_2024-10-30_00:42:52.914.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYifan Hong
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090; scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF-X; Bruker Daltonics amaZon series
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-30 00:39:03ID requested
12024-10-30 00:42:53announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: chronic lead exposure, thiol metabolism, immunotoxicity, CD4+ T, neutrophil
Contact List
Yifan Hong
contact affiliationShenzhen Bay Laboratory
contact emailangivan@163.com
lab head
Yifan Hong
contact affiliationShenzhen Bay Laboratory
contact emailangivan@163.com
dataset submitter
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