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PXD057337

PXD057337 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteins raw data using CT-1 in MDA-MB-231
DescriptionThe MDA-MB-231 cells were treated with CT-1 for 24 h. Then the cells were collected for proteomic analysis. SDT buffer was used for sample lysis and protein extraction. Protein digestion by trypsin was performed according to filter-aided sample preparation (FASP) procedure described by Matthias Mann. Then 100 μg peptide mixture of each sample was labeled using TMT reagent according to the manufacturer’s instructions. LC-MS/MS analysis was performed on a Q Exactive mass spectrometer that was coupled to Easy nLC for 60/90 min. The MS raw data for each sample were searched using the MASCOT engine embedded into Proteome Discoverer 1.4 software for identification and quantitation analysis.
HostingRepositoryiProX
AnnounceDate2024-10-30
AnnouncementXMLSubmission_2024-10-29_23:08:23.188.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLijun Zhang
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-29 23:07:59ID requested
12024-10-29 23:08:23announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Proteins raw data, CT-1, MDA-MB-231 cells
Contact List
Xin Luan
contact affiliationShanghai University of Traditional Chinese Medicine
contact emailluanxin@shutcm.edu.cn
lab head
Lijun Zhang
contact affiliationShanghai University of Traditional Chinese Medicine
contact emailzhanglijun0407@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI