PXD057337

PXD057337 is an original dataset announced via ProteomeXchange.

Title	Proteins raw data using CT-1 in MDA-MB-231
Description	The MDA-MB-231 cells were treated with CT-1 for 24 h. Then the cells were collected for proteomic analysis. SDT buffer was used for sample lysis and protein extraction. Protein digestion by trypsin was performed according to filter-aided sample preparation (FASP) procedure described by Matthias Mann. Then 100 μg peptide mixture of each sample was labeled using TMT reagent according to the manufacturer’s instructions. LC-MS/MS analysis was performed on a Q Exactive mass spectrometer that was coupled to Easy nLC for 60/90 min. The MS raw data for each sample were searched using the MASCOT engine embedded into Proteome Discoverer 1.4 software for identification and quantitation analysis.
HostingRepository	iProX
AnnounceDate	2024-10-30
AnnouncementXML	Submission_2025-08-21_19:00:07.122.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lijun Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-29 23:07:59	ID requested
1	2024-10-29 23:08:23	announced
⏵ 2	2025-08-21 19:00:07	announced	2025-08-22: Add info.

Liu Y, Sun Q, Guo J, Yan L, Yan Y, Gong Y, Lin J, Yuan H, Jin J, Wang B, Chen H, Zhang L, Zhang W, Luan X, Dual ferroptosis induction in N2-TANs and TNBC cells via FTH1 targeting: A therapeutic strategy for triple-negative breast cancer. Cell Rep Med, 6(1):101915(2025) [pubmed]

submitter keyword: Proteins raw data, CT-1, MDA-MB-231 cells

Xin Luan
contact affiliation	Shanghai University of Traditional Chinese Medicine
contact email	luanxin@shutcm.edu.cn
lab head
Lijun Zhang
contact affiliation	Shanghai University of Traditional Chinese Medicine
contact email	zhanglijun0407@163.com
dataset submitter

iProX dataset URI