PXD057319

PXD057319 is an original dataset announced via ProteomeXchange.

Title	Activity based proteome profiling of serum serine hydrolases: application in pediatric abusive head trauma
Description	Purpose: Traumatic brain injury (TBI), including pediatric abusive head trauma (AHT), is the leading cause of death and disability in children and young adults worldwide. The current understanding of trauma-induced molecular changes in the brain of human subjects with intracranial haemorrhage (ICH) remains inadequate and requires further investigation to improve the outcome and management of TBI in the clinic. Calcium-mediated damage at the site of brain injury has been shown to activate several catalytic enzymes. Experimental design: Serine hydrolases (SHs) are major catalytic enzymes involved in the biochemical pathways of blood coagulation, systemic inflammation and neuronal signaling. Here we investigated activity-based protein profiling (ABPP) by measuring the activity status of SH enzymes in the serum of infants with severe ICH as a consequence of AHT or atraumatic infants who died of sudden infant death syndrome (SIDS). Results: Our proof-of-principle study revealed significantly reduced physiological activity of dozens of metabolic SHs in the serum of infants with severe AHT compared to the SIDS group, with some of the enzymes being related to neurodevelopment and basic brain metabolism.
HostingRepository	PRIDE
AnnounceDate	2025-06-26
AnnouncementXML	Submission_2025-06-26_14:26:54.419.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tatjana Sajic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	10-fluoroethoxyphosphinyl-N-(biotinamidopentyl)decanamide; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-29 15:48:34	ID requested
⏵ 1	2025-06-26 14:26:55	announced

Maret E, Wiskott K, Shipley T, Gilardi F, Augsburger M, Thomas A, Fracasso T, Sajic T, Activity-Based Proteome Profiling of Serum Serine Hydrolases: Application in Pediatric Abusive Head Trauma. Proteomics Clin Appl, 19(2):e202400022(2025) [pubmed]

10.1002/prca.202400022;

submitter keyword: Human, serum , Activity based proteome profilling

Tatjana Sajic
contact affiliation	University Center of Legal Medicine, Lausanne University Hospital, Geneva University Hospital, Switzerland
contact email	Tatjana.Sajic@chuv.ch
lab head
Tatjana Sajic
contact affiliation	University of Lausanne
contact email	tatjana.sajic@chuv.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057319
     1. Label: PRIDE project
     2. Name: Activity based proteome profiling of serum serine hydrolases: application in pediatric abusive head trauma