PXD057318

PXD057318 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of small extracellular vesicles derived from human retinal Müller glial cells under mannitol control and high glucose in vitro
Description	Small extracellular vesicles (sEVs) are critical for cell-to-cell communication in retinal health and disease. This study aims to characterize the proteome of sEVs derived from human retinal Müller glial cells under normal glucose and high glucose conditions to understand their role in diabetic retinopathy. We performed high-resolution proteomic analyses on sEVs and their parent retinal Müller glial cells under both normal glucose and high glucose conditions. Comprehensive bioinformatics analyses, including gene ontology and protein-protein interaction network analyses, were conducted to assess the biological processes, molecular functions, cellular components, and signaling pathways associated with differentially expressed proteins. Additionally, physical attributes of sEVs, such as particle size, concentration, and morphology, were evaluated. Our analysis revealed no significant differences in the physical attributes of sEVs between normal glucose and high glucose conditions. However, proteomic analysis identified substantial alterations in the protein composition of sEVs under high glucose conditions. Differentially expressed proteins included common sEV proteins as well as cell-specific proteins. Gene ontology analysis highlighted enriched biological processes, molecular functions, cellular components, and signaling pathways associated with these differentially expressed proteins. Protein-protein interaction network analysis illustrated the interaction networks of differentially expressed proteins and identified specific functional modules within these networks. The main conclusions are that sEVs derived from retinal Müller glial cells transmit altered protein constituents under diabetic conditions, contributing to the pathogenesis of diabetic retinopathy.
HostingRepository	PRIDE
AnnounceDate	2025-09-11
AnnouncementXML	Submission_2025-09-11_13:58:34.045.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thao Thi Nguyen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-29 15:45:39	ID requested
⏵ 1	2025-09-11 13:58:34	announced

Dataset with its publication pending

submitter keyword: diabetic retinopathy

extracellular vesicles

exosomes

proteomics

retina

metabolism

RNA binding protein

Müller glial cells

Huang, Hu
contact affiliation	University of Missouri
contact email	huangh1@health.missouri.edu
lab head
Thao Thi Nguyen
contact affiliation	University of Missouri-Columbia
contact email	tnguyen24@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD057318

PRIDE project URI

• PRIDE
  1. PXD057318
     1. Label: PRIDE project
     2. Name: Proteomics analysis of small extracellular vesicles derived from human retinal Müller glial cells under mannitol control and high glucose in vitro