PXD057294 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The role and cooperation of AidB dehydrogenase and AlkB dioxygenase in repair of adducts to DNA and RNA bases |
Description | The aim of the project is to conduct in vivo, in vitro and in silico studies of the substrate specificity of AidB dehydrogenase against the by-products rising in AlkB dioxygenase repair reaction and AlkB and AidB cooperative interaction in the repair of adducts to DNA and RNA bases. AidB dehydrogenase and AlkB dioxygenase are induced in the E. coli adaptive response to alkylating agents. As a result of direct AlkB removal of base modifications, toxic and mutagenic by-products are formed: formaldehyde, glyoxal or malondialdehyde. Our studies indicate that AlkB is efficiently inhibited in vitro by the side products of the repair reaction. The biological role and substrates of AidB are unknown. It is known that AidB does not repair DNA lesions; its crystallographic structure points to small molecule substrates. This allowed me to formulate the hypothesis that AidB dehydrogenase may be involved in the detoxification of highly reactive by-products generated as a result of AlkB repair and that to prevent their release to the cell, these enzymes can interact directly in the cell homeostasis maintenance. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_04:49:44.448.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ewa Sitkiewicz |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-10-29 07:40:17 | ID requested | |
⏵ 1 | 2025-05-07 04:49:45 | announced | |
Publication List
10.1021/acs.jproteome.4c00700; |
Pucha, ł, a W, Kistowski M, Zhukova L, Burdukiewicz M, Dadlez M, HRaDeX: R Package and Web Server for Computing High-Resolution Deuterium Uptake Rates for HDX-MS Data. J Proteome Res, 24(4):1688-1700(2025) [pubmed] |
Keyword List
submitter keyword: AidB dehydrogenase, DNA repair, AlkB dioxygenase, E.coli, RNA repair |
Contact List
Michał Dadlez |
contact affiliation | Institute of Biochemistry and Biophysics, Pol. Acad. Sci. Mass Sepctrometry Laboratory, Head |
contact email | michald@ibb.waw.pl |
lab head | |
Ewa Sitkiewicz |
contact affiliation | Mass Spectrometry Laboratory
Institute of Biochemistry and Biophysics Polish Academy of Sciences |
contact email | ewa@ibb.waw.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD057294 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD057294
- Label: PRIDE project
- Name: The role and cooperation of AidB dehydrogenase and AlkB dioxygenase in repair of adducts to DNA and RNA bases