PXD057291

PXD057291 is an original dataset announced via ProteomeXchange.

Title	Adipose tissue-derived microRNAs as epigenetic modulators of type 2 diabetes
Description	White adipose tissue (WAT) dysfunction along with an aberrant expression of miRNAs are strongly associated with the risk of developing type 2 diabetes (T2D) with limited evidence linking early changes in the WAT-derived miRNAs and T2D. The present study aims to identify early miRNome changes prognostic for T2D in mice and humans. Gonadal (g) WAT of diabetes-resistant and -prone mice were subjected to multi-omics analyses (transcriptome, miRNome, methylome, proteome). Metabolic phenotypes linked with T2D were correlated with adipose tissue miRNA expression and DNA methylation from 14 monozygotic twin pairs discordant for T2D. Plasma miRNA levels from females at high risk of developing T2D (TÜF study) were included. Adipose tissue of the diabetes-susceptible mice was less insulin sensitive with 150 differentially expressed miRNAs compared to diabetes-resistant mice. Integrative analysis of miRNome-transcriptome-proteome identified 61 proteins involved in actin cytoskeleton, amino acid and sphingolipid metabolism. More than 20 miRNAs are located in the imprinted region Dlk1-Gtl2 and miR-335 in Mest and regulated by DNA methylation. Imprinted miRNAs also exhibited similar alteration in monozygotic twin pairs discordant for T2D with miR-335 expression altered only in females. Moreover, plasma levels of miR-335-5p were negatively correlated with fasting blood glucose in female individuals at high risk of developing T2D. Early alterations of WAT-derived miRNAs such as miR-335-5p could contribute to systemic metabolic changes associated with the risk of developing T2D.
HostingRepository	PRIDE
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-14_16:59:20.342.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fiorella Andrea Solari
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	time-of-flight

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-29 07:23:04	ID requested
⏵ 1	2025-12-14 16:59:21	announced

Sehgal R, Haacke N, Maguolo A, Solari FA, J, ä, hnert M, Gottmann P, Nilsson E, Vaag A, Fischer-Posovszky P, Werberger A, Birkenfeld AL, Fritsche A, H, ä, ring HU, Sickmann A, Vogel H, Ling C, Ouni M, Sch, ü, rmann A, Adipose tissue-derived microRNAs as epigenetic modulators of type 2 diabetes. BMC Med, 23(1):678(2025) [pubmed]

10.1186/s12916-025-04560-7;

submitter keyword: proteomics,Adipose tissue, Data indipendent acquistion, diabetes

Albert Sickmann
contact affiliation	Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
contact email	sickmann@isas.de
lab head
Fiorella Andrea Solari
contact affiliation	Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
contact email	fiorella.solari@isas.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD057291

PRIDE project URI

• PRIDE
  1. PXD057291
     1. Label: PRIDE project
     2. Name: Adipose tissue-derived microRNAs as epigenetic modulators of type 2 diabetes