PXD057281

PXD057281 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomics effects of exposure of fatty acids in the human liver cell line Huh7
Description	Non-alcoholic fatty liver disease (NAFLD), also referred to metabolic associated fatty liver disease (MAFLD), displays characteristics indicative of metabolic disorders. Despite extensive research efforts, the underlying pathogenesis of NAFLD remain largely unknown and currently lack recommended treatment options. It is widely acknowledged that obesity and type 2 diabetes are significant risk factors for NAFLD, with the accumulation of free fatty acids (FFA) in the liver considered an initial trigger for its development. In this study, we exposed the human liver cell line Huh7 to a high concentration of a mixture containing both saturated (palmitic acid) and unsaturated (oleic acid) fatty acids for 24 hours. TMT and LC-MS based proteomics yielded a set of 172 significantly regulated proteins.
HostingRepository	PRIDE
AnnounceDate	2026-03-16
AnnouncementXML	Submission_2026-03-16_06:13:34.428.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johan Palmfeldt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-29 04:32:59	ID requested
⏵ 1	2026-03-16 06:13:35	announced

Publication List

10.1186/s12876-026-04658-z;
Zhou T, Zhou Y, C, ö, mert C, Zhou XY, Lin L, Bolund L, Palmfeldt J, Luo Y, Bross P, Tong G, Energy metabolism alteration and gene expression reprogramming in a cell model of high fat load non-alcoholic fatty liver disease. BMC Gastroenterol, 26(1):(2026) [pubmed]

10.1186/s12876-026-04658-z;

Zhou T, Zhou Y, C, ö, mert C, Zhou XY, Lin L, Bolund L, Palmfeldt J, Luo Y, Bross P, Tong G, Energy metabolism alteration and gene expression reprogramming in a cell model of high fat load non-alcoholic fatty liver disease. BMC Gastroenterol, 26(1):(2026) [pubmed]

Keyword List

submitter keyword: Cell Models
NAFLD, TMT,Liver cells, Energy metabolism

submitter keyword: Cell Models

NAFLD, TMT,Liver cells, Energy metabolism

Contact List

Johan Palmfeldt
contact affiliation	Aarhus University, Denmark
contact email	johan.palmfeldt@clin.au.dk
lab head
Johan Palmfeldt
contact affiliation	Aarhus University Hospital
contact email	johan.palmfeldt@clin.au.dk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD057281

PRIDE project URI

Repository Record List

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