PXD057200

PXD057200 is an original dataset announced via ProteomeXchange.

Title	Mg2+-dependent mechanism of environmental versatility in a multidrug efflux pump
Description	Tripartite resistance nodulation and cell division multidrug efflux pumps span the periplasm and are major drivers of multidrug resistance among Gram-negative bacteria. Cations, such as Mg2+, become concentrated within the periplasm and, in contrast to the cytoplasm, it’s pH is sensitive to conditions outside the cell. Here, we reveal an interplay between Mg2+ and pH in modulating the structural dynamics of the periplasmic adaptor protein, AcrA, and its function within the prototypical AcrAB-TolC multidrug pump from Escherichia coli. In the absence of Mg2+, AcrA becomes increasingly plastic within acidic conditions, but when Mg2+ is bound this is ameliorated, resulting instead in domain specific organisation. We establish a unique histidine residue directs these dynamics and is essential for sustaining pump activity across acidic, neutral, and basic regimes. Overall, we propose Mg2+ conserves AcrA structural mobility to ensure optimal AcrAB-TolC function within rapid changing environments commonly faced during bacterial infection and colonization.
HostingRepository	PRIDE
AnnounceDate	2024-12-30
AnnouncementXML	Submission_2024-12-30_08:52:45.699.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Russell Lewis
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Xevo G2 Q-Tof; Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-25 08:01:40	ID requested
⏵ 1	2024-12-30 08:52:46	announced

10.1016/J.STR.2024.12.012;

submitter keyword: Hydrogen/deuteruim exchange mass spectrometry, molecular dynamics simualtions,Antibiotic resistance, Multidrug efflux pumps

Eamonn Reading
contact affiliation	Associate Professor, School of Biological Sciences, University of Southampton
contact email	E.Reading@soton.ac.uk
lab head
Benjamin Russell Lewis
contact affiliation	King's College London
contact email	benjamin.russell_lewis@kcl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057200
     1. Label: PRIDE project
     2. Name: Mg2+-dependent mechanism of environmental versatility in a multidrug efflux pump