PXD057163
PXD057163 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | YME1L1 Degrades Mitochondria Protein Import Complex TIMM17A and TIMM23 Subunits and Protects Cells upon Import Plugging |
| Description | Mitochondrial protein import through the outer and inner membranes is key to mitochondrial biogenesis. Recent studies have explored how cells respond when import is impaired by a variety of different insults. Here, we developed a mammalian import blocking system using dihydrofolate reductase (DHFR) fused to the N-terminus of the inner membrane protein MIC60. While stabilization of the DHFR domain by methotrexate inhibited endogenous mitochondrial protein import, it neither activated the transcription factor ATF4, nor was affected by ATAD1 expression or by VCP/p97 inhibition. On the other hand, surprisingly, plugging the channel of translocase of the outer membrane (TOM) induced YME1L1, an ATP-dependent protease, to eliminate translocase of the inner membrane (TIM23) channel components TIMM17A and TIMM23. The data suggest that unoccupied TIM23 complexes expose a C-terminal degron on TIMM17A to YME1L1 for degradation. Import plugging caused a cell growth defect and loss of YME1L1 exacerbated the growth inhibition, showing the protective effect of YME1L1 activity. YME1L1 appears to play a crucial role in mitochondrial quality control to counteract precursor stalling in the TOM complex and unoccupied TIM23 channels. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-11-01 |
| AnnouncementXML | Submission_2024-11-01_12:23:49.349.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Linlin Lei |
| SpeciesList | scientific name: Human; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-10-24 14:39:34 | ID requested | |
| ⏵ 1 | 2024-11-01 12:23:49 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: YME1L1, mitochondria import, TIMM17A, TIMM23, DatasetType:Proteomics |
Contact List
| Meng-Chieh Hsu | |
|---|---|
| contact affiliation | Department of Animal Science and Technology, National Taiwan University |
| contact email | mengchiehhsu@gmail.com |
| lab head | |
| Koji Yamano | |
| contact affiliation | Department of Biomolecular Pathogenesis, Medical Research Institute, Tokyo Medical and Dental University |
| contact email | kojibiom@tmd.ac.jp |
| lab head | |
| Richard youle | |
| contact affiliation | Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health |
| contact email | youler@ninds.nih.gov |
| lab head | |
| Linlin Lei | |
| contact affiliation | NIH |
| contact email | Linlin.Lei@nih.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000096201/ |
to receive all new ProteomeXchange dataset release announcements!
