PXD057161

PXD057161 is an original dataset announced via ProteomeXchange.

Title	The quantitative proteomics analysis of TGF-β induced EMT in A549 cells and TNS-overexpressing A549 cells
Description	Metastasis is the primary cause of mortality in lung cancer patients, with the epithelial-mesenchymal transition (EMT) process conferring increased metastatic potential to carcinoma cells. While emerging evidence suggests that TNS1 contributes to carcinoma metastasis, it remains unclear the role of TNS1 in EMT. In this study, we reveal TNS1 as an indispensable factor in TGF-β and hypoxia mediated EMT process. Elevated TNS1 protein levels are closely correlated with a worse prognosis in lung cancer patients. TNS1 is also found to potently promote EMT process and tumor metastasis in vitro and in vivo. Mechanistically, TNS1 expression is mediated by the transcription factor SMAD3 in TGF-β signaling. Moreover, TNS1 interacts with and modulates ZEB1 expression, preventing ZEB1 ubiquitination degradation. Therefore, our study reveals the role of TNS1 in the EMT process and suggests that TNS1 promises to a candidate for targeted cancer therapy.
HostingRepository	PRIDE
AnnounceDate	2025-12-08
AnnouncementXML	Submission_2025-12-07_16:32:47.490.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tingting Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-24 12:21:59	ID requested
⏵ 1	2025-12-07 16:32:48	announced

Zhang T, Li Z, Ming Y, Li J, Ye Z, Luo D, Wang R, Yang Y, Yang X, Peng Y, -induced epithelial-to-mesenchymal transition in lung cancer. Commun Biol, 8(1):1686(2025) [pubmed]

10.1038/s42003-025-09079-4;

submitter keyword: Epithelial-mesenchymal transition (EMT), TNS1,Lung cancer, SMAD3, ZEB1

Yong Peng
contact affiliation	Advanced Mass Spectrometry Center, Research Core Facility, Frontiers Scienc Center for Disease-Related Molecular Network, West China Hospital
contact email	ttcpu2012@126.com
lab head
Tingting Zhang
contact affiliation	Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
contact email	ttcpu2012@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD057161

PRIDE project URI

• PRIDE
  1. PXD057161
     1. Label: PRIDE project
     2. Name: The quantitative proteomics analysis of TGF-β induced EMT in A549 cells and TNS-overexpressing A549 cells