PXD057158

PXD057158 is an original dataset announced via ProteomeXchange.

Title	Metaproteomics reveals age-specific alterations of gut microbiome in hamsters with SARS-CoV-2 infection
Description	The gut microbiome's pivotal role in health and disease is well-established. SARS-CoV-2 infection often causes gastrointestinal symptoms and is associated with changes of the microbiome in both human and animal studies. While hamsters serve as important animal models for coronavirus research, there exists a notable void in the functional characterization of their microbiomes with metaproteomics. In this study, we present a workflow for analyzing the hamster gut microbiome, including a metagenomics-derived hamster gut microbial protein database and a data-independent acquisition metaproteomics method. Using this workflow, we identified 32419 protein groups from the fecal microbiomes of young and old hamsters infected with SARS-CoV-2. We showed age-specific changes in the expressions of microbiome functions and host proteins associated with microbiomes, providing further functional insight into the dysbiosis and aberrant cross-talks between the microbiome and host in SARS-CoV-2 infection. Altogether this study established and demonstrated the capability of metaproteomics for the study of hamster microbiomes.
HostingRepository	PRIDE
AnnounceDate	2025-05-13
AnnouncementXML	Submission_2025-05-13_07:35:08.541.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marybeth Creskey
SpeciesList	scientific name: Mesocricetus auratus (Golden hamster); NCBI TaxID: 10036;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-24 10:45:27	ID requested
⏵ 1	2025-05-13 07:35:08	announced

10.1080/19490976.2025.2505117;

submitter keyword: metaproteomics, gut microbiome, data-independent acquisition, COVID-19

Xu Zhang
contact affiliation	Regulatory Research Division, Biologic and Radiopharmaceutical Drugs Directorate, Health Products and Food Branch, Health Canada, Ottawa K1A 0K9, Canada
contact email	xu.zhang@hc-sc.gc.ca
lab head
Marybeth Creskey
contact affiliation	Health Canada
contact email	marybeth.creskey@hc-sc.gc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD057158

PRIDE project URI

• PRIDE
  1. PXD057158
     1. Label: PRIDE project
     2. Name: Metaproteomics reveals age-specific alterations of gut microbiome in hamsters with SARS-CoV-2 infection