PXD057152

PXD057152 is an original dataset announced via ProteomeXchange.

Title	Role of Late Endosomal Autophagy-Dependent Secretion in Regulating ER and Mitochondrial Stress and Stress Communication
Description	Late endosomal secretion is an unconventional mechanism that relies on VAMP7 and is upregulated in autophagy-null cells. Transcriptomic analysis of VAMP7 knockout (KO) cells revealed significant changes in the expression of genes related to the mitochondria and endoplasmic reticulum (ER) stress. We observed major morphological and functional mitochondrial defects in VAMP7KO cells both in vitro and in vivo in mouse models. Specifically, VAMP7KO cells exhibited impaired transport of mitochondrial-derived vesicles to late endosomes and reduced mitochondrial component secretion. Similarly, VAMP7 was involved in membrane trafficking from the ER to late endosomes and secretion of ER components. Pharmacological induction of stress in the ER or mitochondria led to an increased release of their respective components, in a manner dependent on both VAMP7 and autophagy. In an in vivo glioblastoma model, grafting VAMP7 KO cells into rat brains resulted in larger, more necrotic tumors with higher macrophage infiltration, whereas autophagy-null cells induced smaller and less necrotic tumors. In conclusion, late endosomal secretion plays a crucial role in maintaining ER and mitochondrial quality control and regulates tumor growth and necrosis, thus late endosomal autophagy-dependent secretion is a quality control and a stress communication mechanism.
HostingRepository	PRIDE
AnnounceDate	2026-01-26
AnnouncementXML	Submission_2026-01-26_03:59:41.773.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara guerrera
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-24 05:28:40	ID requested
⏵ 1	2026-01-26 03:59:42	announced

Dataset with its publication pending

submitter keyword: mitochondrail stress,autophagy, Vamp7

Thierry Galli
contact affiliation	Université Paris Cité, Institute of Psychiatry and Neuroscience of Paris, INSERM U1266 102-108 rue de la Santé 75014 Paris France
contact email	thierry.galli@inserm.fr
lab head
Chiara guerrera
contact affiliation	Necker proteomics, INSERM
contact email	chiara.guerrera@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD057152

PRIDE project URI

• PRIDE
  1. PXD057152
     1. Label: PRIDE project
     2. Name: Role of Late Endosomal Autophagy-Dependent Secretion in Regulating ER and Mitochondrial Stress and Stress Communication