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PXD057151

PXD057151 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOrofiamma_AMPK_Arf6_TripleTOF6600_P116
DescriptionThis dataset consists of 18 raw MS files and associated peak lists and results files, acquired on a ABSCiex TripleTOF6600 operated in Data Dependent Acquisition mode. Sample generation and affinity purification was performed by Geoffrey Hesketh. Mass spectrometry acquisition was performed by Geoffrey Hesketh. Analysis was performed by Geoffrey Hesketh, Laura Orofiamma, Anne-Claude Gingras, and Costin Antonescu. The files are associated with a manuscript submitted for publication by Laura Orofiamma et al. This dataset identifies proximity-dependent interactions of ARF6 in wildtype, GDP-locked and GTP-locked states using BioID in HeLa cells. The associated manuscript identifies AMPK as a regulator of cargo-specific clathrin-dependent endocytosis dynamics, which acts through ARF6 regulation. Costin Antonescu is the corresponding author of the manuscript (cantonescu@torontomu.ca); Anne-Claude Gingras should be contacted for questions on this dataset (gingras@lunenfeld.ca) This submission is associated with 2 Supplementary Files (in addition to this README file) Table 1 describes the composition of this dataset Table 2 lists all the peptide identification evidence Table 3 lists the SAINTexpress interactions
HostingRepositoryMassIVE
AnnounceDate2025-06-06
AnnouncementXMLSubmission_2025-06-06_05:32:50.420.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterGingras Lab
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListdeamidated L-asparagine; deamidated L-glutamine; L-methionine sulfoxide
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-24 05:25:44ID requested
12025-06-06 05:32:50announced
Publication List
no publication
Keyword List
submitter keyword: BioID, Proximity-dependent biotinylation, Arf6, AMPK, clathrin-dependent endocytosis, DatasetType:Proteomics
Contact List
Anne-Claude Gingras
contact affiliationLTRI
contact emailgingras@lunenfeld.ca
lab head
Gingras Lab
contact affiliationSamuel Lunenfeld Research Institute
contact emailgingras@mshri.on.ca
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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