PXD057149 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mesenchymal Colorectal Cancers Secrete Vesicles With Unique Cargo That Can Be Used For Liquid Biopsy Based Diagnostics |
| Description | Tumor-derived extracellular vesicles (TEVs) play a crucial role in cancer progression, metastasis and therapy resistance but their distinct profiles across different cancer stages and molecular subtypes remain underexplored. This study analyzed TEVs from epithelial (CMS2) and mesenchymal (CMS4) subtypes of colorectal cancer (CRC) using six cell lines and clinical samples. Investigation of the cargo of vesicles secreted by the two subtypes revealed significant differences in mRNA, miRNA, and protein profiles between the two subtypes. Notably, CMS2 predominantly secreted smaller, Tetraspanin-8 (TSPAN8) enriched EVs, while CMS4 produced both larger and smaller EVs, enriched in TSPAN4. This underscores the complexity of vesicle heterogeneity between these subtypes. Additionally, we assessed miRNA profiles from plasma-derived bulk TEVs in CRC patients. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-10 |
| AnnouncementXML | Submission_2025-11-09_16:52:22.771.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Thang Pham |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-10-24 05:23:26 | ID requested | |
| ⏵ 1 | 2025-11-09 16:52:23 | announced | |
Publication List
| 10.1002/jev2.70171; |
| Asif PJ, Borghuis LH, van Hooff SR, Grootemaat AE, van Eijndhoven MAJ, de Rooij J, Groenewegen NJ, Boza JP, Kranenburg O, Rinkes IHMB, G, ó, mez-Mart, í, n C, Pruijt HFM, van der Wel NN, Torang A, Buffart TE, Pegtel DM, Medema JP, Mesenchymal Colorectal Cancers Secrete Vesicles With Unique Cargo That Can Be Used for Liquid Biopsy Based Diagnostics. J Extracell Vesicles, 14(11):e70171(2025) [pubmed] |
Keyword List
| submitter keyword: tumor-derived extracellular vesicles, proteomic analysis, TSPAN4, diagnostic tool, miRNA signature,Colorectal cancer, consensus molecular subtypes, TSPAN8 |
Contact List
| Connie Jimenez |
|---|
| contact affiliation | Amsterdam UMC, Vrije Universiteit Amsterdam, Medical Oncology, Cancer Center Amsterdam, OncoProteomics Laboratory, Amsterdam, Netherlands |
| contact email | c.jimenez@amsterdamumc.nl |
| lab head | |
| Thang Pham |
|---|
| contact affiliation | Amsterdam UMC |
| contact email | t.pham@amsterdamumc.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD057149 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD057149
- Label: PRIDE project
- Name: Mesenchymal Colorectal Cancers Secrete Vesicles With Unique Cargo That Can Be Used For Liquid Biopsy Based Diagnostics
