PXD057128

PXD057128 is an original dataset announced via ProteomeXchange.

Title	Probing the Role of O-GlcNAcylation in Modulating Protein-DNA Interactions: Insights from a Proteomic Transcription Factor Response Element-Based Pulldown Screening Approach
Description	O-linked β-N-acetylglucosamine (O-GlcNAc), a critical post-translational modification (PTM) predominantly found in the nucleus, plays a substantial role in regulating gene expression by modulating transcription factors (TFs) activity. Herein, by utilizing a pulldown screening approach termed as tandem array of transcription factors response elements (catTFREs), we unravel the profound impact of fluctuating levels of O-GlcNAcylation on the DNA binding efficiency of endogenous TFs. The proteins were enriched for label-free quantitative proteomics by LC-MS/MS.
HostingRepository	iProX
AnnounceDate	2024-10-24
AnnouncementXML	Submission_2025-08-21_19:05:28.638.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yubo Liu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-23 20:10:26	ID requested
1	2024-10-23 20:10:48	announced
⏵ 2	2025-08-21 19:05:29	announced	2025-08-22: Add info.

Li G, Meng F, Zhong X, Yu K, Zhang N, Zhang K, Huang H, Li W, Zhang J, Wang W, Ren Y, Liu Y, DNA probe pulldown screening uncovers O-GlcNAcylation modulation of transcription factor DNA interactions. Sci Rep, 15(1):22679(2025) [pubmed]

submitter keyword: O-GlcNAc, transcription factors, DNA-protein interaction

Yubo Liu
contact affiliation	Dalian University of Technology
contact email	liuyubo@dlut.edu.cn
lab head
Yubo Liu
contact affiliation	Dalian University of Technology
contact email	liuyubo@dlut.edu.cn
dataset submitter

iProX dataset URI