PXD057122

PXD057122 is an original dataset announced via ProteomeXchange.

Title	Proteome profiling followng targeting eIF4E
Description	Here we have compared the impact of loss the protein synthesis initiation factor eIF4e in non samll cell lung cancer cells. Expression of eIF4E was targeted by an eIF4E siRNA or by expressiojn of eIF4E-dTAG construct allowing degradation of eIF4e-dTAG with the heterobifunctional dTAGv-1 small molecule. Proteomes were profiled following 72 hr exposure of the four H1299 eIF4E-dTAG clones (C2-2, C3-1, N2-1 and N3-2) to 0.5 microM dTAGV-1 or H1299 parent cells transfected with 1 microg eIF4E siRNA (siTOOLS, Planegg, Germany) using Lipofectamine 3000 transfection reagent (Fisher Scientific, cat#L3000008).
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_04:29:50.690.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Clarke
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-23 18:44:21	ID requested
⏵ 1	2025-05-07 04:29:51	announced

10.1038/s41467-024-54356-1;

Sharp SY, Martella M, D'Agostino S, Milton CI, Ward G, Woodhead AJ, Richardson CJ, Carr MG, Chiarparin E, Cons BD, Coyle J, East CE, Hiscock SD, Martinez-Fleites C, Mortenson PN, Palmer N, Pathuri P, Powers MV, Saalau SM, St Denis JD, Swabey K, Vinkovi, ć M, Walton H, Williams G, Clarke PA, Integrating fragment-based screening with targeted protein degradation and genetic rescue to explore eIF4E function. Nat Commun, 15(1):10037(2024) [pubmed]

submitter keyword: Human, cancer, lung, dTAG, protein synthesis, eIF4E, siRNA

Paul Andrew Clarke
contact affiliation	Institute of Cancer Research
contact email	paul.clarke@icr.ac.uk
lab head
Paul Clarke
contact affiliation	Centre for Cancer Drug Discovery, Institute of Cancer Research
contact email	paul.clarke@icr.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD057122

PRIDE project URI

• PRIDE
  1. PXD057122
     1. Label: PRIDE project
     2. Name: Proteome profiling followng targeting eIF4E