PXD057113

PXD057113 is an original dataset announced via ProteomeXchange.

Title	Mass Spectrometry Imaging and ScRNA-seq Techniques to Explore the Biosynthetic pathways of Bufadienolides
Description	Bufotoxin is an endogenous toxin made up of several physiologically active components that toads deploy as a defense against their natural enemies. Bufadienolides (BDS), which is isolated from bufotoxin, is an important anticancer drug, and other components such as bufotenine and alkaloids are also important drug resources. The distribution characteristics and biosynthesis of bufotoxins in the postauricular glands (PGs) of toads are not well understood. We examined the toad's PGs using the MADLI/MSI technique, a total of 1,872 components were found, and some pharmacological components were visible. These findings indicate that bufotoxins are primarily abundant in the plasma glands (pG) and epidermal tissues of the glands. By using single-cell sequencing, it was possible to create a single-cell atlas of 9316 PGs cells. These cells were then categorized into nine clusters using marker genes, and two types of epithelial cells were verified using in situ hybridization investigations. It was confirmed that cholesterol is a precursor component of BDS biosynthesis, we concentrated on the cholesterol metabolism component and postulated the primary bile acid pathway as a downstream biosynthesis pathway of BDS through transcriptomic studies of two pG and mucous glands (MG) with distinct secretory functions. Optimal and silenced genes for potential BDS synthesis pathways, toad toxin tryptamine and alkaloid biosynthesis, terpene skeleton and steroid hormones were identified by calculating the cellular coverage of genes. Our data demonstrate the metabolic mapping of bufotoxins in the PGs of the toad, and create the first single-cell atlas of PGs in the toad, providing a reference for the study of biosynthesis of natural active ingredients in animals.
HostingRepository	PRIDE
AnnounceDate	2024-11-05
AnnouncementXML	Submission_2024-11-05_07:37:32.200.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhang shilin
SpeciesList	scientific name: Bufo gargarizans andrewsi; NCBI TaxID: 61428;
ModificationList	No PTMs are included in the dataset
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-23 10:52:26	ID requested
⏵ 1	2024-11-05 07:37:32	announced

Dataset with its publication pending

submitter keyword: Bufo gargarizans

postauricular gland

Bufotoxin

bufadienolides

MALDI/MSI

scRNA-seq

biosynthetic pathway

Jihai Gao
contact affiliation	State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China
contact email	gaojihai@cdutcm.edu.cn
lab head
zhang shilin
contact affiliation	State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China.
contact email	2523223182@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD057113

PRIDE project URI

• PRIDE
  1. PXD057113
     1. Label: PRIDE project
     2. Name: Mass Spectrometry Imaging and ScRNA-seq Techniques to Explore the Biosynthetic pathways of Bufadienolides