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PXD057096

PXD057096 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMechanistic Insight into Human Milk Extracellular Vesicle-Intestinal Barrier Interactions
DescriptionHuman milk extracellular vesicles (EVs) are crucial mother-to-baby messengers that transfer biological signals. These EVs are reported to survive digestion and transport across the intestine. The mechanisms of interaction between human milk EVs and the intestinal mucosa, including epithelial uptake remain unclear. Here, we studied the interaction of human milk EVs with the gut barrier components, including intestinal biofluids, enzymes, mucus and epithelium. Additionally, we probed the endocytic mechanisms mediating the EV intestinal uptake. Finally, using proteomic analysis, we determined the existence and identification of proteins enriched in the EV fraction transported across the intestinal epithelium. We show that human milk EVs are largely stable in the biochemical gut barriers and demonstrate high mucus diffusivity. EVs show a high level of epithelial cell uptake (~70%), and efficient transport across Caco-2 monolayers. Whilst cell uptake of EVs was mediated by multiple routes, none of the pathway-specific inhibitors inhibited their epithelial translocation. Proteomic analysis of EVs transported across Caco-2 monolayers identified 14 enriched EV proteins that may facilitate intestinal transport. These findings significantly expand our understanding of the interactions between human milk EVs and the gut barriers, including their intestinal uptake.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_03:59:32.263.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXiang Luo
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-23 03:32:31ID requested
12025-05-07 03:59:33announced
Publication List
Luo X, Zhang Y, Ding N, Javorovic J, Raimi-Abraham BT, Lynham S, Yang X, Shenker N, Vllasaliu D, Mechanistic insight into human milk extracellular vesicle-intestinal barrier interactions. J Extracell Biol, 4(1):e70032(2025) [pubmed]
10.1002/jex2.70032;
Keyword List
submitter keyword: EV intestinal translocation, EV cell uptake,Human milk EVs, EV proteomics
Contact List
Driton Vllasaliu
contact affiliationInstitute of Pharmaceutical Science, School of Cancer and Pharmaceutical Science,United Kingdom
contact emaildriton.vllasaliu@kcl.ac.uk
lab head
Xiang Luo
contact affiliationKing's College London
contact emailk1778511@kcl.ac.uk
dataset submitter
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Dataset FTP location
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PRIDE project URI
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