PXD057096 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mechanistic Insight into Human Milk Extracellular Vesicle-Intestinal Barrier Interactions |
Description | Human milk extracellular vesicles (EVs) are crucial mother-to-baby messengers that transfer biological signals. These EVs are reported to survive digestion and transport across the intestine. The mechanisms of interaction between human milk EVs and the intestinal mucosa, including epithelial uptake remain unclear. Here, we studied the interaction of human milk EVs with the gut barrier components, including intestinal biofluids, enzymes, mucus and epithelium. Additionally, we probed the endocytic mechanisms mediating the EV intestinal uptake. Finally, using proteomic analysis, we determined the existence and identification of proteins enriched in the EV fraction transported across the intestinal epithelium. We show that human milk EVs are largely stable in the biochemical gut barriers and demonstrate high mucus diffusivity. EVs show a high level of epithelial cell uptake (~70%), and efficient transport across Caco-2 monolayers. Whilst cell uptake of EVs was mediated by multiple routes, none of the pathway-specific inhibitors inhibited their epithelial translocation. Proteomic analysis of EVs transported across Caco-2 monolayers identified 14 enriched EV proteins that may facilitate intestinal transport. These findings significantly expand our understanding of the interactions between human milk EVs and the gut barriers, including their intestinal uptake. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_03:59:32.263.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xiang Luo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-10-23 03:32:31 | ID requested | |
⏵ 1 | 2025-05-07 03:59:33 | announced | |
Publication List
Luo X, Zhang Y, Ding N, Javorovic J, Raimi-Abraham BT, Lynham S, Yang X, Shenker N, Vllasaliu D, Mechanistic insight into human milk extracellular vesicle-intestinal barrier interactions. J Extracell Biol, 4(1):e70032(2025) [pubmed] |
10.1002/jex2.70032; |
Keyword List
submitter keyword: EV intestinal translocation, EV cell uptake,Human milk EVs, EV proteomics |
Contact List
Driton Vllasaliu |
contact affiliation | Institute of Pharmaceutical Science, School of Cancer and Pharmaceutical Science,United Kingdom |
contact email | driton.vllasaliu@kcl.ac.uk |
lab head | |
Xiang Luo |
contact affiliation | King's College London |
contact email | k1778511@kcl.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD057096
- Label: PRIDE project
- Name: Mechanistic Insight into Human Milk Extracellular Vesicle-Intestinal Barrier Interactions