PXD057096

PXD057096 is an original dataset announced via ProteomeXchange.

Title	Mechanistic Insight into Human Milk Extracellular Vesicle-Intestinal Barrier Interactions
Description	Human milk extracellular vesicles (EVs) are crucial mother-to-baby messengers that transfer biological signals. These EVs are reported to survive digestion and transport across the intestine. The mechanisms of interaction between human milk EVs and the intestinal mucosa, including epithelial uptake remain unclear. Here, we studied the interaction of human milk EVs with the gut barrier components, including intestinal biofluids, enzymes, mucus and epithelium. Additionally, we probed the endocytic mechanisms mediating the EV intestinal uptake. Finally, using proteomic analysis, we determined the existence and identification of proteins enriched in the EV fraction transported across the intestinal epithelium. We show that human milk EVs are largely stable in the biochemical gut barriers and demonstrate high mucus diffusivity. EVs show a high level of epithelial cell uptake (~70%), and efficient transport across Caco-2 monolayers. Whilst cell uptake of EVs was mediated by multiple routes, none of the pathway-specific inhibitors inhibited their epithelial translocation. Proteomic analysis of EVs transported across Caco-2 monolayers identified 14 enriched EV proteins that may facilitate intestinal transport. These findings significantly expand our understanding of the interactions between human milk EVs and the gut barriers, including their intestinal uptake.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:59:32.263.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiang Luo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-23 03:32:31	ID requested
⏵ 1	2025-05-07 03:59:33	announced

Luo X, Zhang Y, Ding N, Javorovic J, Raimi-Abraham BT, Lynham S, Yang X, Shenker N, Vllasaliu D, Mechanistic insight into human milk extracellular vesicle-intestinal barrier interactions. J Extracell Biol, 4(1):e70032(2025) [pubmed]

10.1002/jex2.70032;

submitter keyword: EV intestinal translocation, EV cell uptake,Human milk EVs, EV proteomics

Driton Vllasaliu
contact affiliation	Institute of Pharmaceutical Science, School of Cancer and Pharmaceutical Science,United Kingdom
contact email	driton.vllasaliu@kcl.ac.uk
lab head
Xiang Luo
contact affiliation	King's College London
contact email	k1778511@kcl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD057096

PRIDE project URI

• PRIDE
  1. PXD057096
     1. Label: PRIDE project
     2. Name: Mechanistic Insight into Human Milk Extracellular Vesicle-Intestinal Barrier Interactions