PXD057080

PXD057080 is an original dataset announced via ProteomeXchange.

Title	Effects of moderate intensity exercise on liver metabolism in mice based on multi-omics analysis
Description	Background: Physical exercise is beneficial to keep physical and mental health. The molecular mechanisms underlying exercise are still worth exploring. Methods: The healthy adult mice after six weeks of moderate-intensity exercise (experimental group) and sedentary mice (control group) were used to perform transcriptomic, proteomic, lactylation modification, and metabolomics analysis. In addition, gene sets related to hypoxia, glycolysis, and fatty acid metabolism were used to aid in the screening of hub genes. The mMCP-counter was employed to evaluate infiltration of immune cells in murine liver tissues. Results: Transcriptomics analysis revealed 82 intersection genes related to hypoxia, glycolysis, and fatty acid metabolism. Proteomics and lactylation modification analysis identified 577 proteins and 141 differentially lactylation modification proteins. By overlapping 82 intersection genes with 577 differentially expressed proteins and 141 differentially lactylation modification proteins, three hub genes (Aldoa, Acsl1, and Hadhb) were obtained. The immune infiltration analysis revealed a decreased score for monocytes/macrophages and an increased score for endothelial cells in the experimental group. Then, 459 metabolites in positive mode and 181 metabolites in negative mode were identified. The “Metabolic pathways” (mmu01100) was a common pathway between intersection genes-enriched pathways and metabolites-enriched pathways. Conclusion: These findings highlight the pivotal roles of hub genes in the glycolysis and fatty acid metabolism under the context of chronic exercise.
HostingRepository	PRIDE
AnnounceDate	2025-02-10
AnnouncementXML	Submission_2025-02-09_18:17:17.425.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wanyu Wu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	lactic acid
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-22 20:41:51	ID requested
⏵ 1	2025-02-09 18:17:17	announced

10.1038/S41598-024-82150-Y;

submitter keyword: lactylation, multi-omics, metabolome, liver, proteome,lchronic exercise

Jiahui Chang, Wanyu Wu, Ping Qian, Zhaoxu Lu, Xuejia He, Fang Wang, Ting Zhang
contact affiliation	Peking Union Medical College
contact email	zhangting_cp@163.com
lab head
Wanyu Wu
contact affiliation	Peking Union Medical College
contact email	wuwanyu0474230@sina.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057080
     1. Label: PRIDE project
     2. Name: Effects of moderate intensity exercise on liver metabolism in mice based on multi-omics analysis