PXD057061

PXD057061 is an original dataset announced via ProteomeXchange.

Title	Beyond RNA Modification: A Novel Role for tRNA modifying Enzyme in Oxidative Stress Response
Description	RNA modifications are fundamental to regulating essential cellular processes, including RNA folding, stability, gene expression, and the accuracy and efficiency of decoding. These modifications are added post-transcriptionally by specialized enzymes, yet their broader roles beyond RNA modification remain poorly understood. In this study, we uncovered a novel function for Vibrio cholerae tRNA dihydrouridine synthase B (VcDusB) in bacterial adaptation to oxidative stress. While VcDusB is traditionally recognized for catalyzing the reduction of uridine to dihydrouridine in tRNA, our findings revealed that its deletion severely compromises V. cholerae survival under hydrogen peroxide-induced oxidative stress. Unexpectedly, VcDusB not only modulated codon decoding efficiency at its target tRNAs, but also influenced global translation, including codons unrelated to its canonical substrates—a phenomenon particularly evident under oxidative stress conditions. Proteomic analysis of the ∆dusB strain exposed a substantial reduction in protein synthesis, highlighting a widespread translational defect in response to stress. Strikingly, point mutations in DusB demonstrated that its protective role during stress was independent of its tRNA modification activity. Instead, its ability to bind tRNA and its NADPH oxidase activity proved vital for survival. These findings reveal a previously unrecognized, non-canonical function of DusB in oxidative stress defense, expanding our understanding of RNA-modifying enzymes. More broadly, this work paves the way for investigating the multifaceted roles of these enzymes in cellular metabolism and stress responses, far beyond their established functions in RNA modification
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_18:57:46.731.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magalie Duchateau
SpeciesList	scientific name: Vibrio cholerae O1 biovar El Tor; NCBI TaxID: 686;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-22 09:43:00	ID requested
⏵ 1	2025-08-24 18:57:47	announced

10.1016/j.jmb.2025.169107;

de Cr, é, cy-Lagard V, Baharoglu Z, Yuan Y, Bo, ë, l G, Babor J, Bacusmo JM, Dedon PC, Ho P, Hummels KR, Kearns D, Are Bacterial Processes Dependent on Global Ribosome Pausing Affected by tRNA Modification Defects? J Mol Biol, 437(16):169107(2025) [pubmed]

submitter keyword: RNA modification, Vibrio cholerae,oxidative stress

Zeynep BAHAROGLU
contact affiliation	Institut Pasteur Unité "Plasticité du Génome Bactérien" Département Génomes et Génétique 28 rue du Docteur Roux 75724 Paris Cedex 15
contact email	zeynep.baharoglu@pasteur.fr
lab head
Magalie Duchateau
contact affiliation	Institut Pasteur
contact email	magalie.duchateau@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD057061

PRIDE project URI

• PRIDE
  1. PXD057061
     1. Label: PRIDE project
     2. Name: Beyond RNA Modification: A Novel Role for tRNA modifying Enzyme in Oxidative Stress Response