PXD057054

PXD057054 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Human cartilage model of the precocious osteoarthritis-inducing COL2A1 p.R719C reveals pathology-driving matrix defects and a failure of the ER proteostasis network to recognize the defective procollagen-II
Description	10-plex TMT dataset generated by immunoprecipitation of intracellular, epitope-tagged COL2A1 constructs along with DSP-crosslinked interactors. This experiment allows for the identification and quantitative comparison of wild-type and Arg719Cys COL2A1 interactomes in HT-1080 cells transiently transfected with COL2A1 under a constitutive promoter. Negative control samples are untransfected HT-1080 cells, used to identify high-confidence collagen-II interactors from non-specific binders.
HostingRepository	MassIVE
AnnounceDate	2025-05-30
AnnouncementXML	Submission_2025-05-30_11:55:30.950.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kathryn Yammine
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-22 06:09:18	ID requested
⏵ 1	2025-05-30 11:55:31	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: COL2A1, interactome, quantitative proteomics, TMT, DatasetType:Proteomics

submitter keyword: COL2A1, interactome, quantitative proteomics, TMT, DatasetType:Proteomics

Contact List

Matthew Shoulders
contact affiliation	MIT
contact email	mshoulde@mit.edu
lab head
Kathryn Yammine
contact affiliation	MIT
contact email	kyammine@mit.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v07/MSV000096151/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v07/MSV000096151/