PXD057048

PXD057048 is an original dataset announced via ProteomeXchange.

Title	Raw data sets for Identification of OsHSP70 as a key regulator of MSP1-induced signaling in rice
Description	Magnaporthe oryzae snodprot1 homolog (MSP1), a recently identified PAMP, is known to trigger defense responses in rice. However, the receptor(s) and downstream interacting components of MSP1 in rice have not been identified so far. Using multiple immunoprecipitation-mass spectrometry (IP-MS) analyses in transgenic rice expressing MSP1-YFP, we identified several heat shock proteins (HSPs) as MSP1 interacting partners. Heat stress treatment suppressed MSP1-induced cell death in tobacco plants transiently expressing MSP1. Subsequent IP-MS analysis on these tobacco plants, exposed to heat stress, resulted in the identification of a HSP having a highly conserved sequence to the OsHSP70 of rice. Co-expression of MSP1 with OsHSP70 failed to induce cell death in tobacco, confirming the involvement of the OsHSP70 in the MSP1 immune response. Rice protoplasts, co-expressing MSP1 and OsHSP70, showed a direct interaction between these two proteins in planta. Taken together, the results reported here highlight the pivotal role of OsHSP70 in MSP1-induced signaling and enhance our understanding of rice-M. oryzae interaction.
HostingRepository	PRIDE
AnnounceDate	2025-04-23
AnnouncementXML	Submission_2025-04-22_23:48:14.014.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sun Tae Kim
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-22 00:27:44	ID requested
⏵ 1	2025-04-22 23:48:14	announced

10.1007/S00344-025-11648-0;

submitter keyword: MSP1,Magnaporthe oryzae, IP-MS, Heat shock protein, Cell death

Sun Tae Kim
contact affiliation	Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, 50463, Republic of Korea
contact email	stkim71@pusan.ac.kr
lab head
Sun Tae Kim
contact affiliation	Pusan National University
contact email	stkim5505@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057048
     1. Label: PRIDE project
     2. Name: Raw data sets for Identification of OsHSP70 as a key regulator of MSP1-induced signaling in rice