PXD057021

PXD057021 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of natural photoheterotrophic mixed consortium for biohydrogen production under nongrowing conditions
Description	A biohydrogen and polyhydroxyalkanoates(PHA)-producing natural photoheterotrophic mixed culture composed mainly by Rhodopseudomonas palustris and Clostridium sp was studied by a proteomic analysis under non-growth conditions (nitrogen-absence and organic acids). Proteins in C. pasteurianum were upregulated, particularly those related to stress response. In contrast, C. pasteurianum in the consortium did not present such proteins, showing the advantage of being part of it. Both cultures showed proteins involved in organic acid metabolism and biohydrogen production, such as lactate dehydrogenase, ferredoxins, and hydrogenases. Proteomes of R. palustris as single culture and in consortium showed that organic acids were redirect into central carbon pathways to generate reduced equivalents for biohydrogen production. Light-harvesting proteins and fatty acid metabolism linked to PHA accumulation were also upregulated. This study provides insights into how the proteomes of individual organisms and their consortium counterparts adapt to non-growth conditions, shedding light on how microbial interactions influence protein expression.
HostingRepository	PRIDE
AnnounceDate	2025-02-06
AnnouncementXML	Submission_2025-02-05_23:02:52.161.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Timothy McCubbin
SpeciesList	scientific name: Clostridium pasteurianum DSM 525 = ATCC 6013; NCBI TaxID: 1262449; scientific name: Rhodopseudomonas palustris; NCBI TaxID: 1076;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-21 10:13:16	ID requested
⏵ 1	2025-02-05 23:02:52	announced

10.1016/J.BIORTECH.2024.132023;

submitter keyword: Proteins expression, bioH2, Clostridium sp., microbial consortia, organic acids, photofermentation, Rhodopseudomonas palustris

Elvia Ines Garcia Pena
contact affiliation	Instituto Politécnico Nacional, Mexico, Mexico
contact email	egarciap@ipn.mx
lab head
Timothy McCubbin
contact affiliation	University of Queensland
contact email	t.mccubbin@uq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD057021

PRIDE project URI

• PRIDE
  1. PXD057021
     1. Label: PRIDE project
     2. Name: Proteomic analysis of natural photoheterotrophic mixed consortium for biohydrogen production under nongrowing conditions