PXD057012

PXD057012 is an original dataset announced via ProteomeXchange.

Title	PERK crosstalk between ER and microtubule dynamics
Description	The architecture of the endoplasmic reticulum (ER) is tightly controlled as a key determinant of its function. Its dynamics are linked to those of the cytoskeleton, but our understanding of how this coordination occurs and what its functional relevance is, is limited. We found the Unfolded Protein Response (UPR) transducer EIF2AK3/PERK is essential for acute stress-induced peripheral redistribution and remodeling of the ER, through eIF2a phosphorylation and translation initiation shutdown. PERK-mediated eIF2a phosphorylation can be bypassed by blocking polysome assembly; by depleting microtubule-anchoring ER proteins such as REEP4, p180/RRBP1 and Climp63/CKAP4; or by disrupting the microtubule cytoskeleton. Notably, specific disruption of non-centrosomal microtubules, but not centrosome depletion, relieved blockade of ER redistribution in PERK-deficient cells. Conversely, PERK deficiency stabilized non-centrosomal microtubules promoting polarized protrusiveness in epithelial cells and neuroblasts. Thus, PERK coordinates ER architecture and homeostasis with cell morphogenesis by coupling ER remodeling and non-centrosomal MT stability and dynamics.
HostingRepository	PRIDE
AnnounceDate	2025-11-03
AnnouncementXML	Submission_2025-11-02_16:51:55.924.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan A Lopez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-21 06:28:04	ID requested
⏵ 1	2025-11-02 16:51:56	announced

S, á, nchez-, Á, lvarez M, Lolo FN, Sailem H, Fulgoni G, Pascual-Vargas P, Ag, ü, era L, Catal, á, -Montoro M, Arias-Garc, í, a M, L, ó, pez JA, V, á, zquez J, Del Pozo M Á, Bakal C, PERK-dependent reciprocal crosstalk between ER and non-centrosomal microtubules coordinates ER architecture and cell shape. Cell Rep, 44(5):115590(2025) [pubmed]

10.1016/j.celrep.2025.115590;

submitter keyword: Human, endoplasmic reticulum (ER) , Unfolded Protein Response

Juan A Lopez
contact affiliation	Proteomics Unit, CNIC Madrid, Spain
contact email	jalopez@cnic.es
lab head
Juan A Lopez
contact affiliation	CNIC
contact email	jalopez@cnic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD057012

PRIDE project URI

• PRIDE
  1. PXD057012
     1. Label: PRIDE project
     2. Name: PERK crosstalk between ER and microtubule dynamics