PXD057005

PXD057005 is an original dataset announced via ProteomeXchange.

Title	Legionella effector LpPIP recruits protein phosphatase 1 to the mitochondria to induce dephosphorylation of outer membrane proteins
Description	Legionella pneumophila utilises a type IVB secretion system (T4SS) to translocate over 300 effectors into host cells, hijacking cellular processes, including those within the mitochondrion. Currently, no Legionella effectors have been identified at the mitochondrial outer membrane, a critical interface between the organelle and rest of the cell. We screened the Legionella effector repertoire for features of mitochondrial tail-anchored (TA) proteins and identified four putative TA effectors. Among them, Lpg1625 was confirmed to localize to the mitochondrial outer membrane and shown to interact with all three isoforms of protein phosphatase 1 (PP1) through an RVxF motif. Importantly, PP1 remains catalytically active upon interaction with Lpg1625, leading to dephosphorylation of specific mitochondrial outer membrane proteins. Altering the TA signature to direct Lpg1625 to the ER led to ER-recruitment of PP1 and subsequent dephosphorylation of ER resident proteins indicating that Lpg1625 controls PP1 localisation and not substrate specificity. This study uncovers a novel pathogen-mediated strategy to modulate PP1 and manipulate the host phosphoproteome.
HostingRepository	PRIDE
AnnounceDate	2025-06-03
AnnouncementXML	Submission_2025-06-03_05:54:09.969.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kai Qi Yek
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse; Orbitrap Exploris 480; Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-21 03:14:20	ID requested
⏵ 1	2025-06-03 05:54:10	announced

Dataset with its publication pending

submitter keyword: mitochondrial outer membrane, protein phosphatase 1, Legionella pneumophila, effector proteins,mitochondria, tail-anchored protein, RVxF motif, type IVB secretion system

Diana Stojanovski
contact affiliation	Department of Biochemistry and Pharmacology, The University of Melbourne, Parkville, 3052, Victoria, Australia
contact email	d.stojanovski@unimelb.edu.au
lab head
Kai Qi Yek
contact affiliation	University of Melbourne
contact email	kyek@student.unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD057005

PRIDE project URI

• PRIDE
  1. PXD057005
     1. Label: PRIDE project
     2. Name: Legionella effector LpPIP recruits protein phosphatase 1 to the mitochondria to induce dephosphorylation of outer membrane proteins