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PXD057005

PXD057005 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLegionella effector LpPIP recruits protein phosphatase 1 to the mitochondria to induce dephosphorylation of outer membrane proteins
DescriptionLegionella pneumophila utilises a type IVB secretion system (T4SS) to translocate over 300 effectors into host cells, hijacking cellular processes, including those within the mitochondrion. Currently, no Legionella effectors have been identified at the mitochondrial outer membrane, a critical interface between the organelle and rest of the cell. We screened the Legionella effector repertoire for features of mitochondrial tail-anchored (TA) proteins and identified four putative TA effectors. Among them, Lpg1625 was confirmed to localize to the mitochondrial outer membrane and shown to interact with all three isoforms of protein phosphatase 1 (PP1) through an RVxF motif. Importantly, PP1 remains catalytically active upon interaction with Lpg1625, leading to dephosphorylation of specific mitochondrial outer membrane proteins. Altering the TA signature to direct Lpg1625 to the ER led to ER-recruitment of PP1 and subsequent dephosphorylation of ER resident proteins indicating that Lpg1625 controls PP1 localisation and not substrate specificity. This study uncovers a novel pathogen-mediated strategy to modulate PP1 and manipulate the host phosphoproteome.
HostingRepositoryPRIDE
AnnounceDate2025-06-03
AnnouncementXMLSubmission_2025-06-03_05:54:09.969.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKai Qi Yek
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentOrbitrap Eclipse; Orbitrap Exploris 480; Orbitrap Ascend
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-21 03:14:20ID requested
12025-06-03 05:54:10announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mitochondrial outer membrane, protein phosphatase 1, Legionella pneumophila, effector proteins,mitochondria, tail-anchored protein, RVxF motif, type IVB secretion system
Contact List
Diana Stojanovski
contact affiliationDepartment of Biochemistry and Pharmacology, The University of Melbourne, Parkville, 3052, Victoria, Australia
contact emaild.stojanovski@unimelb.edu.au
lab head
Kai Qi Yek
contact affiliationUniversity of Melbourne
contact emailkyek@student.unimelb.edu.au
dataset submitter
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Dataset FTP location
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PRIDE project URI
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