PXD057005 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Legionella effector LpPIP recruits protein phosphatase 1 to the mitochondria to induce dephosphorylation of outer membrane proteins |
Description | Legionella pneumophila utilises a type IVB secretion system (T4SS) to translocate over 300 effectors into host cells, hijacking cellular processes, including those within the mitochondrion. Currently, no Legionella effectors have been identified at the mitochondrial outer membrane, a critical interface between the organelle and rest of the cell. We screened the Legionella effector repertoire for features of mitochondrial tail-anchored (TA) proteins and identified four putative TA effectors. Among them, Lpg1625 was confirmed to localize to the mitochondrial outer membrane and shown to interact with all three isoforms of protein phosphatase 1 (PP1) through an RVxF motif. Importantly, PP1 remains catalytically active upon interaction with Lpg1625, leading to dephosphorylation of specific mitochondrial outer membrane proteins. Altering the TA signature to direct Lpg1625 to the ER led to ER-recruitment of PP1 and subsequent dephosphorylation of ER resident proteins indicating that Lpg1625 controls PP1 localisation and not substrate specificity. This study uncovers a novel pathogen-mediated strategy to modulate PP1 and manipulate the host phosphoproteome. |
HostingRepository | PRIDE |
AnnounceDate | 2025-06-03 |
AnnouncementXML | Submission_2025-06-03_05:54:09.969.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kai Qi Yek |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Eclipse; Orbitrap Exploris 480; Orbitrap Ascend |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-10-21 03:14:20 | ID requested | |
⏵ 1 | 2025-06-03 05:54:10 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: mitochondrial outer membrane, protein phosphatase 1, Legionella pneumophila, effector proteins,mitochondria, tail-anchored protein, RVxF motif, type IVB secretion system |
Contact List
Diana Stojanovski |
contact affiliation | Department of Biochemistry and Pharmacology, The University of Melbourne, Parkville, 3052, Victoria, Australia |
contact email | d.stojanovski@unimelb.edu.au |
lab head | |
Kai Qi Yek |
contact affiliation | University of Melbourne |
contact email | kyek@student.unimelb.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD057005 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD057005
- Label: PRIDE project
- Name: Legionella effector LpPIP recruits protein phosphatase 1 to the mitochondria to induce dephosphorylation of outer membrane proteins