PXD057000

PXD057000 is an original dataset announced via ProteomeXchange.

Title	DDX5 competes with ZC3H12D regulating CCND1 transcript stability to modulate cell cycle progression in breast cancer
Description	This study was performed according to protein pull-down assay using GFP-coated beads in MDA-MB-468 cells expressing GFP/ZC3H12D fusion protein, and then the precipitated proteins were isolated by running SDS-PAGE and silver staining, and identified by high resolution mass spectrometry.This study was performed according to RNA pull-down assay using biotin-labeled WT RNA probe (with stem-loop structure) or mutant RNA probe (without stem-loop structure) and streptavidin-coated Dynabeads in human breast tumor cell MDA-MB-468, and then the pulled down proteins were isolated by SDS-PAGE and silver staining, and identified by high resolution mass spectrometry.
HostingRepository	iProX
AnnounceDate	2024-10-21
AnnouncementXML	Submission_2024-10-21_01:22:32.632.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenbao Lu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-21 01:22:11	ID requested
⏵ 1	2024-10-21 01:22:33	announced

Dataset with its publication pending

submitter keyword: Interacting proteins with ZC3H12D in breast tumor cell MDA-MB-468, protein pull-down followed by MS.Assosiated proteins with CCND1 RNA stem-loop structure in breast tumor cell MDA-MB-468, RNA pull-down followed by MS.

Wenbao Lu
contact affiliation	Chinese Academy of Medical Sciences & Peking Union Medical College
contact email	luwenbao_217@imc.pumc.edu.cn
lab head
Wenbao Lu
contact affiliation	Chinese Academy of Medical Sciences & Peking Union Medical College
contact email	luwenbao_217@imc.pumc.edu.cn
dataset submitter

iProX dataset URI