PXD057000
PXD057000 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | DDX5 competes with ZC3H12D regulating CCND1 transcript stability to modulate cell cycle progression in breast cancer |
Description | This study was performed according to protein pull-down assay using GFP-coated beads in MDA-MB-468 cells expressing GFP/ZC3H12D fusion protein, and then the precipitated proteins were isolated by running SDS-PAGE and silver staining, and identified by high resolution mass spectrometry.This study was performed according to RNA pull-down assay using biotin-labeled WT RNA probe (with stem-loop structure) or mutant RNA probe (without stem-loop structure) and streptavidin-coated Dynabeads in human breast tumor cell MDA-MB-468, and then the pulled down proteins were isolated by SDS-PAGE and silver staining, and identified by high resolution mass spectrometry. |
HostingRepository | iProX |
AnnounceDate | 2024-10-21 |
AnnouncementXML | Submission_2024-10-21_01:22:32.632.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wenbao Lu |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-10-21 01:22:11 | ID requested | |
⏵ 1 | 2024-10-21 01:22:33 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Interacting proteins with ZC3H12D in breast tumor cell MDA-MB-468, protein pull-down followed by MS.Assosiated proteins with CCND1 RNA stem-loop structure in breast tumor cell MDA-MB-468, RNA pull-down followed by MS. |
Contact List
Wenbao Lu | |
---|---|
contact affiliation | Chinese Academy of Medical Sciences & Peking Union Medical College |
contact email | luwenbao_217@imc.pumc.edu.cn |
lab head | |
Wenbao Lu | |
contact affiliation | Chinese Academy of Medical Sciences & Peking Union Medical College |
contact email | luwenbao_217@imc.pumc.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |