PXD056996
PXD056996 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics of Extracellular Vesicles and tissue samples from High-Grade Serous Ovarian Carcinoma |
Description | High-grade serous ovarian carcinoma (HGSOC) is a deadly subtype of ovarian cancer (OC), often diagnosed at late stages due to nonspecific symptoms and lack of effective markers for early detection. Aberrant protein N-linked glycosylation has been reported in HGSOC, holding a potential for improving the diagnosis and prognosis of affected patients. Building on our recent observation documenting that HGSOC-derived extracellular vesicles (EVs) exhibit aberrant protein expression patterns, we here explore the protein N-glycosylation displayed by EVs isolated from HGSOC cell lines and patient sera and ascites relative to those from matching controls to unveil candidate markers for HGSOC. Comparative glycoproteomics of small EVs (sEVs, <200 nm) and medium/large EVs (m/lEVs, >200 nm) isolated from HGSOC and non-cancerous cell lines revealed lower overall N-glycosylation of EV proteins and a decreased protein expression of oligosaccharyltransferase (OST) subunits from HGSOC compared to non-cancerous cell lines. Increased α2,6-sialylation was also observed in m/lEVs from HGSOC cell lines and patient ascites by lectin blotting, which correlated with increased gene expression of ST6GAL1 and decreased gene expression of ST3GAL3-4 in HGSOC compared to normal ovary tissues. Our study provides insights into the EV glycoproteome of HGSOC and the underlying changes in the glycosylation machinery in HGSOC tissues, opening new avenues for the discovery of novel markers against HGSOC. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-04 |
AnnouncementXML | Submission_2025-02-04_00:20:20.735.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rebeca Kawahara |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; complex glycosylation; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-10-20 22:27:19 | ID requested | |
⏵ 1 | 2025-02-04 00:20:21 | announced |
Publication List
Yokoi A, Ukai M, Yasui T, Inokuma Y, Hyeon-Deuk K, Matsuzaki J, Yoshida K, Kitagawa M, Chattrairat K, Iida M, Shimada T, Manabe Y, Chang IY, Asano-Inami E, Koya Y, Nawa A, Nakamura K, Kiyono T, Kato T, Hirakawa A, Yoshioka Y, Ochiya T, Hasegawa T, Baba Y, Yamamoto Y, Kajiyama H, Identifying high-grade serous ovarian carcinoma-specific extracellular vesicles by polyketone-coated nanowires. Sci Adv, 9(27):eade6958(2023) [pubmed] |
10.1016/J.BBADVA.2025.100140; |
10.1126/sciadv.ade6958; |
Keyword List
submitter keyword: glycoproteins, glycoproteomics, extracellular vesicles, ascites,N-glycans, high grade serous carcinoma, biomarker |
Contact List
Rebeca Kawahara | |
---|---|
contact affiliation | Institute for Glyco-core Research (iGCORE), Nagoya University, Aichi, Japan |
contact email | kawahara.sakuma.rebeca.u6@f.mail.nagoya-u.ac.jp |
lab head | |
Rebeca Kawahara | |
contact affiliation | Department of Molecular Sciences, Macquarie University, Sydney, NSW, Australia |
contact email | rebecasakuma@gmail.com |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD056996 |
PRIDE project URI |
Repository Record List
[ + ]