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PXD056996

PXD056996 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics of Extracellular Vesicles and tissue samples from High-Grade Serous Ovarian Carcinoma
DescriptionHigh-grade serous ovarian carcinoma (HGSOC) is a deadly subtype of ovarian cancer (OC), often diagnosed at late stages due to nonspecific symptoms and lack of effective markers for early detection. Aberrant protein N-linked glycosylation has been reported in HGSOC, holding a potential for improving the diagnosis and prognosis of affected patients. Building on our recent observation documenting that HGSOC-derived extracellular vesicles (EVs) exhibit aberrant protein expression patterns, we here explore the protein N-glycosylation displayed by EVs isolated from HGSOC cell lines and patient sera and ascites relative to those from matching controls to unveil candidate markers for HGSOC. Comparative glycoproteomics of small EVs (sEVs, <200 nm) and medium/large EVs (m/lEVs, >200 nm) isolated from HGSOC and non-cancerous cell lines revealed lower overall N-glycosylation of EV proteins and a decreased protein expression of oligosaccharyltransferase (OST) subunits from HGSOC compared to non-cancerous cell lines. Increased α2,6-sialylation was also observed in m/lEVs from HGSOC cell lines and patient ascites by lectin blotting, which correlated with increased gene expression of ST6GAL1 and decreased gene expression of ST3GAL3-4 in HGSOC compared to normal ovary tissues. Our study provides insights into the EV glycoproteome of HGSOC and the underlying changes in the glycosylation machinery in HGSOC tissues, opening new avenues for the discovery of novel markers against HGSOC.
HostingRepositoryPRIDE
AnnounceDate2025-02-04
AnnouncementXMLSubmission_2025-02-04_00:20:20.735.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRebeca Kawahara
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; complex glycosylation; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-20 22:27:19ID requested
12025-02-04 00:20:21announced
Publication List
Yokoi A, Ukai M, Yasui T, Inokuma Y, Hyeon-Deuk K, Matsuzaki J, Yoshida K, Kitagawa M, Chattrairat K, Iida M, Shimada T, Manabe Y, Chang IY, Asano-Inami E, Koya Y, Nawa A, Nakamura K, Kiyono T, Kato T, Hirakawa A, Yoshioka Y, Ochiya T, Hasegawa T, Baba Y, Yamamoto Y, Kajiyama H, Identifying high-grade serous ovarian carcinoma-specific extracellular vesicles by polyketone-coated nanowires. Sci Adv, 9(27):eade6958(2023) [pubmed]
10.1016/J.BBADVA.2025.100140;
10.1126/sciadv.ade6958;
Keyword List
submitter keyword: glycoproteins, glycoproteomics, extracellular vesicles, ascites,N-glycans, high grade serous carcinoma, biomarker
Contact List
Rebeca Kawahara
contact affiliationInstitute for Glyco-core Research (iGCORE), Nagoya University, Aichi, Japan
contact emailkawahara.sakuma.rebeca.u6@f.mail.nagoya-u.ac.jp
lab head
Rebeca Kawahara
contact affiliationDepartment of Molecular Sciences, Macquarie University, Sydney, NSW, Australia
contact emailrebecasakuma@gmail.com
dataset submitter
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Dataset FTP location
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