PXD056984

PXD056984 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of subiculum region during AD pathogenesis
Description	To reveal the pathologic mechanisms involved in large deposits of Aβ amyloid plaques selectively in subiculum region during early stage of AD pathogenesis, high resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed to profile the proteomic changes in different stages of AD. We analyzed the proteome of SUB regions microdissected from wild type (WT) and 5×FAD (AD) mice at 3, 6 and 12 months of age with three biological replicates via data-independent acquisition (DIA) proteomic approach, which had higher stability and coverage and less missing data than label-free quantitative method (LFQ) facilitating identification of low-abundance proteins [1]. In-depth analysis of all replicates using Spectronaut identified 131483 peptides assembling into 8948 protein groups, and more than 4000 proteins was detected in each SUB region, of which 4840 proteins were quantified.
HostingRepository	iProX
AnnounceDate	2024-10-18
AnnouncementXML	Submission_2024-10-20_20:41:10.194.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	He Wang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-20 20:40:37	ID requested
⏵ 1	2024-10-20 20:41:10	announced

Dataset with its publication pending

submitter keyword: Proteome, subiculum, AD pathogenesis

Lihua Zhang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	lihuazhang@dicp.ac.cn
lab head
He Wang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	crane_wanghe@dicp.ac.cn
dataset submitter

iProX dataset URI