PXD056965

PXD056965 is an original dataset announced via ProteomeXchange.

Title	Uncovering the signaling networks of disseminated glioblastoma cells in vivo with INSIGHT
Description	Dysregulation of intracellular signaling networks underpins cancer. Yet, resolving signaling networks within distinct or rare cell types in cancer in vivo has been unattainable. Here we develop INSIGHT by integrating cell sorting with mass spectrometry to enable quantitative phosphoproteomics and proteomics of discrete cell types from fixed tissues. Using INSIGHT, we map the signaling network within disseminating glioblastoma cells from patient-derived xenografts implanted in mice. Disseminating tumor cells undergo a proteome-wide shift from proliferative to mesenchymal, neural progenitor-like cell states. In parallel, signaling network and global kinase activity are rewired, transitioning from cell cycle-associated circuitries to those governing synaptic function, neuronal migration, and ion channel activity. Changes begin at the tumor margin and persist in distant brain parenchyma. Hornerin and phosphorylation of Ca²⁺-permeable GluA2 at Y876 were identified as mediators of glioblastoma progression. INSIGHT enables systems-level dissection of cell-type-specific signaling circuitries in vivo across wide range of biological systems.
HostingRepository	PRIDE
AnnounceDate	2026-05-28
AnnouncementXML	Submission_2026-05-27_23:12:37.787.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD056965
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ryuhjin Ahn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-19 01:12:15	ID requested
⏵ 1	2026-05-27 23:12:38	announced

10.6019/PXD056965;

submitter keyword: None

Forest White
contact affiliation	Massachusetts Institute of Technology
contact email	fwhite@mit.edu
lab head
Ryuhjin Ahn
contact affiliation	McGill University
contact email	ryuhjin.ahn@mcgill.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056965
     1. Label: PRIDE project
     2. Name: Uncovering the signaling networks of disseminated glioblastoma cells in vivo with INSIGHT