PXD056959

PXD056959 is an original dataset announced via ProteomeXchange.

Title	Jade1 and the HBO1 complex are spatial-selective cofactors of Oct4
Description	Oct4 is a master regulator of pluripotency. Potential Oct4 interactors have been cataloged extensively but the manner and significance of these interactions are incompletely defined. Like other POU domain proteins, Oct4 is capable of binding to DNA in multiple configurations, however the relationship between these configurations and cofactor recruitment (and hence transcription output) are unknown. Here, we show that Oct4 interacts with common and unique proteins when bound to DNA in different configurations. One of these proteins is Jade1, a component of the HBO histone acetyltransferase complex. Jade1 preferentially associates with Oct4 when bound to More palindromic Octamer-Related Element (MORE) DNA sequences that bind Oct4 dimers and are associated with strong gene expression. We show that the Oct4 N-terminus is critical for this interaction. ChIP-seq using HBO1, the enzymatic component of the complex, identifies a preference for binding adjacent to Oct4 at MORE sites. Using purified recombinant proteins and nucleosome complexes, we show that the HBO1 complex acetylates histone H3K9 within nucleosomes more efficiently when Oct4 is co-bound to a MORE site. Histone acetylation is further increased when Oct4 is mutated to favor dimeric MORE binding. Cryo-electron microscopy reveals that Oct4 bound to a MORE near the nucleosome entry/exit site partially unwinds DNA from nucleosome core particles, and identifies additional mass associated with the HBO1 complex. These results identify a novel mechanism of transcriptional regulation by Oct4.
HostingRepository	MassIVE
AnnounceDate	2024-11-07
AnnouncementXML	Submission_2024-11-07_13:05:17.381.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yifan Wu
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	methionine oxidation with neutral loss of 80 Da
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-18 14:05:28	ID requested
⏵ 1	2024-11-07 13:05:17	announced

no publication

submitter keyword: Oct4, Cofactor, ESCs, Jade1, MORE motif, Octamer motif, DatasetType:Proteomics

Dean Tantin
contact affiliation	University of Utah
contact email	dean.tantin@path.utah.edu
lab head
Yifan Wu
contact affiliation	University of Utah
contact email	yifan.wu@utah.edu
dataset submitter

MassIVE dataset URI

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