PXD056942

PXD056942 is an original dataset announced via ProteomeXchange.

Title	BPP43_05035 is a Brachyspira pilosicoli cell surface adhesin that weakens the integrity of the epithelial barrier during infection
Description	The anaerobic spirochete Brachyspira causes intestinal spirochetosis, characterized by the intimate attachment of bacterial cells to the colonic mucosa, potentially leading to symptoms such as diarrhea, abdominal pain, and weight loss. Despite the clinical significance of Brachyspira infections, the mechanism of the interaction between Brachyspira and the colon epithelium is not known. We characterized the molecular mechanism of the B. pilosicoli-epithelium interaction and its impact on the epithelial barrier during infection. Through a proteomics approach, we identified BPP43_05035 as a candidate B. pilosicoli surface protein that mediates bacterial attachment to cultured human colonic epithelial cells. The crystal structure of BPP43_05035 revealed a globular lipoprotein with a six-bladed beta-propeller domain. Blocking the native BPP43_05035 on B. pilosicoli, either with a specific antibody or via competitive inhibition, abrogated its binding to epithelial cells, which required cell surface-exposed N-glycans. Proximity labeling and interaction assays revealed that BPP43_05035 bound to tight junctions, thereby increasing the permeability of the epithelial monolayer. Extending our investigation to humans, we discovered a downregulation of tight junction and brush border genes in B. pilosicoli-infected patients carrying detectable levels of epithelium-bound BPP43_05035. Collectively, our findings identify BPP43_05035 as a B. pilosicoli adhesin that weakens the colonic epithelial barrier during infection.
HostingRepository	PRIDE
AnnounceDate	2024-10-18
AnnouncementXML	Submission_2024-10-18_04:51:34.824.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes Fuchs
SpeciesList	scientific name: Brachyspira pilosicoli P43/6/78; NCBI TaxID: 1042417; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-18 04:23:33	ID requested
⏵ 1	2024-10-18 04:51:36	announced

10.1080/19490976.2024.2409247;

10.1016/s0958-3947(01)00066-8;

Rajan A, Gallego P, Dolan B, Patel P, Dwibedi C, Luis AS, Trillo-Muyo S, Arike L, van der Post S, Simr, é, n M, Pelaseyed T, cell surface adhesin that weakens the integrity of the epithelial barrier during infection. Gut Microbes, 16(1):2409247(2024) [pubmed]

Saw CB, Ayyangar KM, Zhen W, Thompson RB, Enke CA, Commissioning and quality assurance for MLC-based IMRT. Med Dosim, 26(2):125-33(2001) [pubmed]

submitter keyword: Brachyspira pilosicoli

Caco-2 cells

IBS

adhesin

intestinal epithelial cells

outer membrane proteins

tight junctions

Thaher Pelaseyed
contact affiliation	Department of Medical Biochemistry and Cell biology, University of Gothenburg, Sweden
contact email	thaher.pelaseyed@medkem.gu.se
lab head
Johannes Fuchs
contact affiliation	Gothenburg University
contact email	johannes.fuchs@gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD056942

PRIDE project URI

• PRIDE
  1. PXD056942
     1. Label: PRIDE project
     2. Name: BPP43_05035 is a Brachyspira pilosicoli cell surface adhesin that weakens the integrity of the epithelial barrier during infection