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PXD056929

PXD056929 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative analysis of the proteome, and protein oxidative modifications, of primary human coronary artery endothelial cells and associated extracellular matrix
DescriptionHere, we characterize the cellular and extracellular matrix proteomes of primary human coronary artery endothelial cells, from multiple donors, and oxidation/nitration products formed on these during cell culture. Liquid chromatography-mass spectrometry with or without prior fractionation, and different data acquisition methods were employed. In total ~9900 proteins were identified across 3 donors, with ~7000 proteins per donor. Of these ~5300 were consistently identified, indicating heterogeneity across the donors, with donor age a probable cause. Multiple endogenous oxidations and nitrations were detected. The former are present on both cell and ECM proteins, whilst a majority of the latter were detected on cell proteins, consistent with intracellular generation of nitrating agents, most likely from eNOS or peroxidase enzyme activity. The modifications are ascribed to both physiological enzymatic activity (hydroxylation at proline/lysine, predominantly on ECM proteins and especially collagens) and reactive species (oxidation at tryptophan/tyrosine/histidine; nitration at tryptophan/tyrosine).
HostingRepositoryPRIDE
AnnounceDate2025-02-17
AnnouncementXMLSubmission_2025-02-17_06:07:26.008.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPer Hägglund
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListnitrated residue; monohydroxylated residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-17 21:13:28ID requested
12025-02-17 06:07:26announced
Publication List
10.1016/J.REDOX.2025.103524;
Keyword List
submitter keyword: nitration, extraceullular matrix, protein oxidation,Endothelial cells
Contact List
Per Hägglund and Mike Davies
contact affiliationUniversity of Copenhagen, Department of Biomedical Sciences
contact emailhagglundperm@gmail.com
lab head
Per Hägglund
contact affiliationUniversity of Copenhagen
contact emailhagglundperm@gmail.com
dataset submitter
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Dataset FTP location
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