PXD056929

PXD056929 is an original dataset announced via ProteomeXchange.

Title	Quantitative analysis of the proteome, and protein oxidative modifications, of primary human coronary artery endothelial cells and associated extracellular matrix
Description	Here, we characterize the cellular and extracellular matrix proteomes of primary human coronary artery endothelial cells, from multiple donors, and oxidation/nitration products formed on these during cell culture. Liquid chromatography-mass spectrometry with or without prior fractionation, and different data acquisition methods were employed. In total ~9900 proteins were identified across 3 donors, with ~7000 proteins per donor. Of these ~5300 were consistently identified, indicating heterogeneity across the donors, with donor age a probable cause. Multiple endogenous oxidations and nitrations were detected. The former are present on both cell and ECM proteins, whilst a majority of the latter were detected on cell proteins, consistent with intracellular generation of nitrating agents, most likely from eNOS or peroxidase enzyme activity. The modifications are ascribed to both physiological enzymatic activity (hydroxylation at proline/lysine, predominantly on ECM proteins and especially collagens) and reactive species (oxidation at tryptophan/tyrosine/histidine; nitration at tryptophan/tyrosine).
HostingRepository	PRIDE
AnnounceDate	2025-02-17
AnnouncementXML	Submission_2025-02-17_06:07:26.008.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Per Hägglund
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	nitrated residue; monohydroxylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-17 21:13:28	ID requested
⏵ 1	2025-02-17 06:07:26	announced

10.1016/J.REDOX.2025.103524;

submitter keyword: nitration, extraceullular matrix, protein oxidation,Endothelial cells

Per Hägglund and Mike Davies
contact affiliation	University of Copenhagen, Department of Biomedical Sciences
contact email	hagglundperm@gmail.com
lab head
Per Hägglund
contact affiliation	University of Copenhagen
contact email	hagglundperm@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056929
     1. Label: PRIDE project
     2. Name: Quantitative analysis of the proteome, and protein oxidative modifications, of primary human coronary artery endothelial cells and associated extracellular matrix