PXD056914

PXD056914 is an original dataset announced via ProteomeXchange.

Title	EXPLORING THE ANTI-INFLAMMATORY POTENTIAL OF THINNED APPLE POLYPHENOLS: MECHANISTIC PROTEOMICS INSIGHTS INTO DNBS-INDUCED COLITIS MODEL IN MICE
Description	Ulcerative colitis (UC) is a multifactorial inflammatory bowel disease (IBD) with increasing incidence worldwide. Current treatments, such as NSAIDs and corticosteroids, provide partial symptom relief but are associated to significant side effects. This highlights the need for novel therapies with fewer adverse effects. Given the role of oxidative stress and inflammation in driving tissue damage during colitis, natural compounds with antioxidant and anti-inflammatory properties are promising therapeutic candidates. We identified thinned apples (TA), an agricultural by-product, as a rich source of polyphenols (TAP) with anti-inflammatory and antioxidant activities in a cell-based model of inflammation. This study evaluates TAP’s therapeutic potential in a DNBS-induced colitis mouse model using label-free quantitative proteomics. Proteomic analysis revealed modulation of several key pathways influenced by TAP treatment, including: (i) activation of antioxidant defence mechanisms; (ii) reversal of DNBS-induced pathways, specifically ferroptosis and heme toxicity; (iii) suppression of immune responses; and (iv) reduction of ulcerative conditions with downregulation of proteins involved in coagulation, inflammation, and angiogenesis. Overall, TAP demonstrated significant therapeutic potential by targeting oxidative stress and inflammation, suggesting its use as a polyphenol-rich extract in health products for UC treatment. Furthermore, utilizing TA as a bioactive extract represents an innovative approach for industrial applications in therapeutic agent production.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_18:02:57.300.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessandra Altomare
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-17 10:48:35	ID requested
⏵ 1	2026-02-22 18:03:00	announced

10.1021/acs.jproteome.5c00653;

Ferrario G, Impellizzeri D, Baron G, D'Amico R, Fumagalli G, Gnasso T, Bombardelli E, Carini M, di Paola R, Aldini G, Altomare A, Proteomics-Driven Mechanistic Insights into the Anti-Inflammatory Potential of Thinned Apple Polyphenols in a DNBS-Induced Colitis Model in Mice. J Proteome Res, 25(2):684-699(2026) [pubmed]

submitter keyword: NF-κB

Nrf2

anti-inflammatory

anti-oxidant

polyphenols

proteomics

thinned apples

Alessandra Altomare
contact affiliation	University of Milan
contact email	alessandra.altomare@unimi.it
lab head
Alessandra Altomare
contact affiliation	University of Milan (DISFARM)
contact email	alessandra.altomare@unimi.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056914
     1. Label: PRIDE project
     2. Name: EXPLORING THE ANTI-INFLAMMATORY POTENTIAL OF THINNED APPLE POLYPHENOLS: MECHANISTIC PROTEOMICS INSIGHTS INTO DNBS-INDUCED COLITIS MODEL IN MICE