PXD056898 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Enhancing ZooMS applications with MALDI-FTICR mass spectrometry on Pleistocene material showing variable collagen preservation |
Description | Advances in high-throughput molecular analyses of collagen peptides, especially ZooMS (Zooarchaeology by Mass Spectrometry), have addressed the issue of intense skeletal fragmentation at Palaeolithic archaeological sites, which hinders morphological identification. However, the challenge of variable collagen preservation persists. We aim to evaluate the potential of two mass analyzers TOF vs FTICR to propose novel options to enhance the ZooMS workflow. Type 1 collagen (COL1) was extracted from 89 archaeological bones from the site of Le Piage (France, 37-34 ka cal BP). Three distinct ZooMS protocols were used: an acid-free method (AmBic) and two demineralizations (HCl and TFA), combined with MALDI-TOF and MALDI-FTICR instruments. The first offers rapid and cheap analysis, while the second provides higher resolution. Taxonomic identifications were made by peptide mass fingerprinting (PMF). Finally, LC-MS/MS was applied to verify 26 low-collagen samples identifications. |
HostingRepository | PRIDE |
AnnounceDate | 2025-03-17 |
AnnouncementXML | Submission_2025-03-17_09:42:30.282.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | BRAY FABRICE |
SpeciesList | scientific name: Rangifer tarandus (Reindeer) (Cervus tarandus); NCBI TaxID: 9870; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; |
ModificationList | monohydroxylated residue; deamidated residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-10-17 04:47:45 | ID requested | |
⏵ 1 | 2025-03-17 09:42:31 | announced | |
Publication List
Raymond P, Ruebens K, Bray F, Castel JC, Morin E, Le Brun-Ricalens F, Bordes JG, Rolando C, Hublin JJ, Using MALDI-FTICR Mass Spectrometry to Enhance ZooMS Identifications of Pleistocene Bone Fragments Showing Variable Collagen Preservation. Rapid Commun Mass Spectrom, ():e10019(2025) [pubmed] |
10.1002/rcm.10019; |
Keyword List
submitter keyword: LC-MSMS, paleoproteomics, PRM, CASI,ZooMS, MALDI-MS, collagen |
Contact List
fabrice bray |
contact affiliation | Laboratoire MSAP UAR 3290 CNRS Bâtiment C4 Avenue Paul Langevin Cité Scientifique 59655 Villeneuve D'ascq 03 20 33 71 12 |
contact email | fabrice.bray@univ-lille.fr |
lab head | |
BRAY FABRICE |
contact affiliation | MSAP USR 3290 |
contact email | fabrice.bray@univ-lille.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD056898 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD056898
- Label: PRIDE project
- Name: Enhancing ZooMS applications with MALDI-FTICR mass spectrometry on Pleistocene material showing variable collagen preservation