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PXD056898

PXD056898 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEnhancing ZooMS applications with MALDI-FTICR mass spectrometry on Pleistocene material showing variable collagen preservation
DescriptionAdvances in high-throughput molecular analyses of collagen peptides, especially ZooMS (Zooarchaeology by Mass Spectrometry), have addressed the issue of intense skeletal fragmentation at Palaeolithic archaeological sites, which hinders morphological identification. However, the challenge of variable collagen preservation persists. We aim to evaluate the potential of two mass analyzers TOF vs FTICR to propose novel options to enhance the ZooMS workflow. Type 1 collagen (COL1) was extracted from 89 archaeological bones from the site of Le Piage (France, 37-34 ka cal BP). Three distinct ZooMS protocols were used: an acid-free method (AmBic) and two demineralizations (HCl and TFA), combined with MALDI-TOF and MALDI-FTICR instruments. The first offers rapid and cheap analysis, while the second provides higher resolution. Taxonomic identifications were made by peptide mass fingerprinting (PMF). Finally, LC-MS/MS was applied to verify 26 low-collagen samples identifications.
HostingRepositoryPRIDE
AnnounceDate2025-03-17
AnnouncementXMLSubmission_2025-03-17_09:42:30.282.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBRAY FABRICE
SpeciesList scientific name: Rangifer tarandus (Reindeer) (Cervus tarandus); NCBI TaxID: 9870; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationListmonohydroxylated residue; deamidated residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-17 04:47:45ID requested
12025-03-17 09:42:31announced
Publication List
Raymond P, Ruebens K, Bray F, Castel JC, Morin E, Le Brun-Ricalens F, Bordes JG, Rolando C, Hublin JJ, Using MALDI-FTICR Mass Spectrometry to Enhance ZooMS Identifications of Pleistocene Bone Fragments Showing Variable Collagen Preservation. Rapid Commun Mass Spectrom, ():e10019(2025) [pubmed]
10.1002/rcm.10019;
Keyword List
submitter keyword: LC-MSMS, paleoproteomics, PRM, CASI,ZooMS, MALDI-MS, collagen
Contact List
fabrice bray
contact affiliation​​Laboratoire MSAP UAR 3290 CNRS Bâtiment C4 Avenue Paul Langevin Cité Scientifique 59655 Villeneuve D'ascq 03 20 33 71 12
contact emailfabrice.bray@univ-lille.fr
lab head
BRAY FABRICE
contact affiliationMSAP USR 3290
contact emailfabrice.bray@univ-lille.fr
dataset submitter
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Dataset FTP location
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