PXD056898

PXD056898 is an original dataset announced via ProteomeXchange.

Title	Enhancing ZooMS applications with MALDI-FTICR mass spectrometry on Pleistocene material showing variable collagen preservation
Description	Advances in high-throughput molecular analyses of collagen peptides, especially ZooMS (Zooarchaeology by Mass Spectrometry), have addressed the issue of intense skeletal fragmentation at Palaeolithic archaeological sites, which hinders morphological identification. However, the challenge of variable collagen preservation persists. We aim to evaluate the potential of two mass analyzers TOF vs FTICR to propose novel options to enhance the ZooMS workflow. Type 1 collagen (COL1) was extracted from 89 archaeological bones from the site of Le Piage (France, 37-34 ka cal BP). Three distinct ZooMS protocols were used: an acid-free method (AmBic) and two demineralizations (HCl and TFA), combined with MALDI-TOF and MALDI-FTICR instruments. The first offers rapid and cheap analysis, while the second provides higher resolution. Taxonomic identifications were made by peptide mass fingerprinting (PMF). Finally, LC-MS/MS was applied to verify 26 low-collagen samples identifications.
HostingRepository	PRIDE
AnnounceDate	2025-03-17
AnnouncementXML	Submission_2025-03-17_09:42:30.282.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	BRAY FABRICE
SpeciesList	scientific name: Rangifer tarandus (Reindeer) (Cervus tarandus); NCBI TaxID: 9870; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-17 04:47:45	ID requested
⏵ 1	2025-03-17 09:42:31	announced

Raymond P, Ruebens K, Bray F, Castel JC, Morin E, Le Brun-Ricalens F, Bordes JG, Rolando C, Hublin JJ, Using MALDI-FTICR Mass Spectrometry to Enhance ZooMS Identifications of Pleistocene Bone Fragments Showing Variable Collagen Preservation. Rapid Commun Mass Spectrom, ():e10019(2025) [pubmed]

10.1002/rcm.10019;

submitter keyword: LC-MSMS, paleoproteomics, PRM, CASI,ZooMS, MALDI-MS, collagen

fabrice bray
contact affiliation	​​Laboratoire MSAP UAR 3290 CNRS Bâtiment C4 Avenue Paul Langevin Cité Scientifique 59655 Villeneuve D'ascq 03 20 33 71 12
contact email	fabrice.bray@univ-lille.fr
lab head
BRAY FABRICE
contact affiliation	MSAP USR 3290
contact email	fabrice.bray@univ-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD056898

PRIDE project URI

• PRIDE
  1. PXD056898
     1. Label: PRIDE project
     2. Name: Enhancing ZooMS applications with MALDI-FTICR mass spectrometry on Pleistocene material showing variable collagen preservation