PXD056880

PXD056880 is an original dataset announced via ProteomeXchange.

Title	Integrative Proteomics and Phosphoproteomics Profiling Reveals Molecular Pathways in hypoxia adaptation of brain tissue in Lasiopodomys brandtii
Description	Exposure to high-altitude hypoxic environments may lead to dysfunction in the central nervous system. Research on the hypoxic response mechanisms of hypoxia-tolerant animals can provide insights for animal conservation and the treatment of ischemic brain injury. In this study, we focused on Brandt's voles and simulated oxygen environments at altitudes of 100m, 3000m, 5000m, and 7000m within a hypoxic chamber. After 24 hours of exposure, brain tissue was collected for quantitative proteomic and phosphoproteomic analyses. A total of 3,947 proteins and 8,572 phosphosites were quantified. Proteomic data revealed that more differentially expressed (DE) proteins were downregulated than upregulated, with significant enrichment in pathways that inhibit protein synthesis and leukocyte activation, suggesting a strategy for energy conservation in Brandt's voles under hypoxic conditions. Additionally, approximately 43% of the DE proteins were identified as candidate genes in other hypoxia-tolerant species. Phosphoproteomic analysis found that phosphoproteins in the downregulated cluster were significantly enriched in vascular smooth muscle contraction and adaptive immune system, which contribute to enhancing oxygen transport to the brain tissue and reducing energy consumption. Furthermore, many DE proteins and phosphoproteins were related to endocytosis, cytoskeleton organization, and synaptic regulation, which may be strategies employed by Brandt's voles to protect brain tissue from hypoxic damage and maintain cognitive function. Utilizing the KSEA algorithm, we predicted that 10 kinase activities in Brandt's voles are significantly influenced by hypoxia, four of which belonging to the CAMK kinase family. Immunoblot validated that the changes in CAMK2A activity were consistent with predictions, suggesting that CAMK may play a crucial role in the hypoxic response. In conclusion, our findings indicate that Brandt's voles cope with hypoxia by enhancing oxygen transport, reducing energy consumption, and activating neuroprotective mechanisms.
HostingRepository	iProX
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2025-09-03_00:26:05.560.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Panqin Wang
SpeciesList	scientific name: Lasiopodomys brandtii; NCBI TaxID: 407171;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-16 20:18:03	ID requested
⏵ 1	2025-09-03 00:26:06	announced

Wang P, Liu Y, Du Y, Gao Y, Shao T, Guo W, Wang Z, Cheng H, ) Brain Tissue. Cells, 14(7):(2025) [pubmed]

submitter keyword: Brain, Hypoxia, Lasiopodomys brandtii, Proteomic, Phosphoproteomic

Han Cheng
contact affiliation	Zhengzhou University
contact email	chenghan@zzu.edu.cn
lab head
Panqin Wang
contact affiliation	Zhengzhou University
contact email	13592571276@163.com
dataset submitter

iProX dataset URI